LOCUS CAD77190.1 1035 aa PRT BCT 27-FEB-2015 DEFINITION Rhodopirellula baltica SH 1 probable multidrug resistance protein protein. ACCESSION BX294152-297 PROTEIN_ID CAD77190.1 SOURCE Rhodopirellula baltica SH 1 ORGANISM Rhodopirellula baltica SH 1 Bacteria; Planctomycetes; Planctomycetia; Pirellulales; Pirellulaceae; Rhodopirellula. REFERENCE 1 (bases 1 to 304250) AUTHORS Gloeckner F.O., Kube M., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Beck A., Borzym K., Heitmann K., Rabus R., Schlesner H., Amann R., Reinhardt R. TITLE Complete genome sequence of the marine planctomycete Pirellula sp. strain 1 JOURNAL Proc. Natl. Acad. Sci. U.S.A. 100(14), 8298-8303(2003). PUBMED 12835416 REFERENCE 2 (bases 1 to 304250) AUTHORS Kube M., Borzym K., Heitmann K., Klages S., Marquardt I., Lehrack S., Beck A., Pawlik R., Reinhardt R., Gloeckner F.O., Bauer M., Teeling H., Lombardot T., Ludwig W., Gade D., Rabus R., Schlesner H., Amann R. JOURNAL Submitted (21-JAN-2003) to the INSDC. Max Planck Institute for Molecular Genetics, proScience Ihnestrasse 73, D-14195 Berlin, Germany Max Planck Institute for Marine Microbiology Celsiusstrasse 1, D-28359 Bremen, Germany. COMMENT This project was carried out by *Max Planck Institute for Molecular Genetics, Berlin, Germany; *Max Planck Institute for Marine Microbiology, Bremen, Germany; in the fremework of the REGX-project, http://www.regx.de -------------- Genome Center Center: Max Planck Institute for Molecular Genetics Center code: MPIMG -------------- Summary Statistics Sequencing vector: pUC19; 100% of reads Chemistry: Dye-terminator Big Dye; 100% of reads Assembly program: Phrap; version 0.990329 Consensus quality: 7142841 bases at least Q40 Consensus quality: 7145138 bases at least Q30 Consensus quality: 7145484 bases at least Q20 Quality coverage: 8.03 -------------- This sequence was finished as follows unless otherwise noted: all regions were double stranded, sequenced with an alternate chemistry, or coveredby high quality data (i.e., phred quality >= 30); an attempt was made to resolve all sequencing problems, such as compressions and repeats; allregions were covered by at least one plasmid Sequence; assembly was additionally confirmed by long range pcr and cosmid end sequences. -------------- See http://www.micro-genomes.mpg.de/pirellula/ for more information including minimal tiling path from a set of 220 cosmids out of 908. See the misc_feature tag below for the boundaries of the MTP cosmids. -------------- Annotation Center: Max Planck Institute for Marine Microbiology Celsiusstrasse 1, D-28359 Bremen, Germany. Center Code: MPIMM Email: fog@mpi-bremen.de Phone: +49 (0)421 2028 938 Fax: +49 (0)421 2028 580 -------------- Three different programs (Glimmer, Critica and Orpheus) were used for ORF-prediction. A nonredundant list of ORFs was generated by suitable parsing of the results. Automated annotation was done with the software package Pedant Pro (http://www.biomax.de). All ORF predictions and annotations were manually corrected by considering all results of the different tools applied. See http://www.regx.de for more information and access to supplementary information. -------------- FEATURES Qualifiers source /organism="Rhodopirellula baltica SH 1" /strain="1" /mol_type="genomic DNA" /db_xref="taxon:243090" protein /transl_table=11 /gene="czcA" /locus_tag="RB11152" /function="resistance proteins; detoxification by export; metal ion transporters (Cu(2+), Fe(2+), etc.)" /note="PMID: 2678100 best DB hits: BLAST: pir:H82300; probable multidrug resistance protein VC0629 [imported] -; E=3e-51 pir:S76433; cation efflux system protein czcA-2 - Synechocystis sp.; E=4e-42 pir:G64595; acriflavine resistance protein - Helicobacter pylori; E=4e-41 COG: VC0629; COG0841 Cation/multidrug efflux pump; E=3e-52 PFAM: PF00873; AcrB/AcrD/AcrF family; E=1e-43" /db_xref="GOA:Q7UJP2" /db_xref="InterPro:IPR001036" /db_xref="InterPro:IPR027463" /db_xref="UniProtKB/TrEMBL:Q7UJP2" BEGIN 1 MTSLSKMVPE GGPIAWMARN SIAANLLMLL LLGGGIWSAF AIQKEVFPQF QLDIVEVAVG 61 YPGAAPEEVE QGILRPIEEA VRGVEGIREV TSEAREGRGE LLIELVGGED RMKVLQDVDQ 121 AVSRIRTFPD QIEQPEVRLQ SRQQEVMQVA IYGPIDVWAL RKLAEQLRDQ LQSKEQITQV 181 ELRRVPAYVT HVEIPRQRLR EYGLTLPDVA EIIRTSSQDV AAGSVQTTAG EILLRVKARK 241 QWAEEFAGIE IVAGRDGPMV TLGDIATIRD GFEEVGFHSQ FSQTPSVELD IYRVGSQSPM 301 DVAEAVQETM EEFETALPPG VKWRIDSNNA EEFRRRLMLV AENAAMAVVI VLVILSLFLE 361 FRLAFWVMMG MAVSFIGGVL LLPAAGVSIN MISLFGFLVV LGIVVDDAVV VGENVYEKRQ 421 TLKDHEAAAV EGTKEVSGPV TFSILTNIVA FVPLLFIPGE TGKFWGPLPV VVIIVLALSL 481 VESLFILPAH LAHARDAGRD PNSIGARLHH LQQRFSQGFN RLVEFFYRPV LILSLRHRYV 541 TASLALALFV VIGGYATSAH MGLILMPEVS ADEIEAGVRM PVGTTQAQAA KIADTVTKAS 601 IKMFEEHNLY EVAEGIKTNV RGQSFIDVEI VMKPPDQRDM TANEVIDLWR EHIGDLPGVN 661 QVTFEAERGP GGHRRDISID LSHSDIDVLE QAATAFVDRV KLYSNARDVN DNYNKGKTQY 721 DFRLRPEGRS LGLTDEELGE QLRGAFFGSL ALRLIRGTNE TEVRVKLPEE QREDIHSLED 781 LIIRTPSGAE VPLLDVAEVE ETLAFRSINR RDGRRTISVS MDVEPKRAAT QVIEALQNSE 841 LPKLREDFPG ITWSFEGSDA EMRQATASLW GSFGLALAVI YSLLAIAFKG YVQPLIVLVA 901 IPFGVVGAII GHIMLGYDLS LVSLMGVIAL SGVVINDSLI MIDYANRHRK NQSAFDAISQ 961 AGLRRFRPIL LTTLTTFGGL MPLIFEDSLQ AQYIIPMAIS LGFGILFATA IILVLVPCLY 1021 LILEDIQKTF TPDTE //