LOCUS CAB5248316.1 478 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 MCE- FAMILY PROTEIN MCE2DA [FIRST PART] protein. ACCESSION LT708304-615 PROTEIN_ID CAB5248316.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="mce2Da" /locus_tag="BQ2027_MB0607" /note="Mb0607, mce2Da, len: 478 aa. Equivalent to 5' end of Rv0592, len: 508 aa, from Mycobacterium tuberculosis strain H37Rv, (99.2% identity in 471 aa overlap). mce2D; belongs to 24-membered Mycobacterium tuberculosis Mce protein family (see citations below for more information), highly similar to Mycobacterium tuberculosis proteins O07416|Rv0172|MTCI28.12|mce1D (530 aa); O53970|Rv1969|MTV051.07|mce3D (423 aa); etc. Also highly similar to others e.g. NP_302659.1|NC_002677 putative secreted protein from Mycobacterium leprae (531 aa); CAC12795.1|AL445327 putative secreted protein from Streptomyces coelicolor (337 aa); etc. Has highly Pro-rich C-terminus and may contain N-terminal signal or anchor sequence. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, mce2D exists as a single gene. In Mycobacterium bovis, a frameshift due to a single base insertion (*-c) splits mce2D into 2 parts, mce2Da and mce2Db. Mb0607 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." BEGIN 1 MSTIFDIRSL RLPKLSAKVV VVGGLVVVLA VVAAAAGARL YRKLTTTTVV AYFSEALALY 61 PGDKVQIMGV RVGSIDKIEP AGDKMRVTLH YSNKYQVPAT ATASILNPSL VASRTIQLSP 121 PYTGGPVLQD GAVIPIERTQ VPVEWDQLRD SINGILRQLG PTERQPKGPF GDLIESAADN 181 LAGKGRQLNE TLNSLSQALT ALNEGRGDFV AITRSLALFV SALYQNDQQF VALNENLAEF 241 TDWFTKSDHD LADTVERIDD VLGTVRKFVS DNRSVLAADV NNLADATTTL VQPEPRDGLE 301 TALHVLPTYA SNFNNLYYPL HSSLVGQFVF PNFANPIQLI CSAIQAGSRL GYQESAELCA 361 QYLAPVLDAL KFNYLPFGSN PFSSAATLPK EVAYSEERLR PPPGYKDTTV PGIFSRDTPF 421 SHGNHEPGWV VAPGMQGMQV QPFTANMLTP ESLAELLGGP DIAPPAAGNQ LARTAECV //