LOCUS CAB5248286.1 286 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE PROTEASE TRANSMEMBRANE PROTEIN HEAT SHOCK PROTEIN HTPX protein. ACCESSION LT708304-585 PROTEIN_ID CAB5248286.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="htpX" /locus_tag="BQ2027_MB0578" /note="Mb0578, htpX, len: 286 aa. Equivalent to Rv0563, len: 286 aa (alternative start at position 654006), from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 286 aa overlap). Probable htpX, protease heat shock protein X (EC 3.4.24.-) (transmembrane protein), equivalent to NP_302484.1|NC_002677 putative peptidase from Mycobacterium leprae (287 aa). Also highly similar to others e.g. CAC08262.1|AL392146 putative peptidase from Streptomyces coelicolor (287 aa); NP_387431.1|NC_003047 PUTATIVE PROTEASE TRANSMEMBRANE PROTEIN from Sinorhizobium meliloti (319 aa); NP_105051.1|NC_002678 heat shock protein (htpX) from Mesorhizobium loti (336 aa); NP_248692.1|NC_000909|U67608|MJU67608_8 heat shock protein HtpX, possibly protease (htpX) from Methanococcus jannaschii (284 aa), FASTA scores: opt: 660, E(): 0, (46.5 identity in 245 aa overlap). Continuation of MTCY25D10.42. TBparse score is 0.887. BELONGS TO PEPTIDASE FAMILY M48 (ZINC METALLOPROTEASE). COFACTOR: Zinc. Protein product from Mb0578 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0578 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTWHPHANRL KTFLLLVGMS ALIVAVGALF GRTALMLAAL FAVGMNVYVY FNSDKLALRA 61 MHAQPVSELQ APAMYRIVRE LATSAHQPMP RLYISDTAAP NAFATGRNPR NAAVCCTTGI 121 LRILNERELR AVLGHELSHV YNRDILISCV AGALAAVITA LANMAMWAGM FGGNRDNANP 181 FALLLVALLG PIAATVIRMA VSRSREYQAD ESGAVLTGDP LALASALRKI SGGVQAAPLP 241 PEPQLASQAH LMIANPFRAG ERIGSLFSTH PPIEDRIRRL EAMARG //