LOCUS       CAB5248276.1             326 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            MUCONATE CYCLOISOMERASE MENC (CIS,CIS-MUCONATE LACTONIZING
            ENZYME) (MLE) protein.
ACCESSION   LT708304-575
PROTEIN_ID  CAB5248276.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="menC"
                     /locus_tag="BQ2027_MB0568"
                     /note="Mb0568, menC, len: 326 aa. Equivalent to Rv0553,
                     len: 326 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 326 aa overlap). Probable menC,
                     muconate cycloisomerase (EC 5.5.1.1), equivalent to
                     NP_302476.1|NC_002677 putative isomerase/racemase from
                     Mycobacterium leprae (334 aa). Also similar to other
                     muconate cycloisomerases e.g. TCBD_PSESP|P27099
                     chloromuconate cycloisomerase (370 aa), FASTA scores: opt:
                     249, E(): 7.8e-09, (32.7% identity in 199 aa overlap).
                     Also similar to O-succinylbenzoate-CoA synthases. BELONGS
                     TO THE MANDELATE RACEMASE / MUCONATE LACTONIZING ENZYME
                     FAMILY. Protein product from Mb0568 detected using shotgun
                     mass spectrometry and SWATH mass spectrometry. Mb0568
                     found to be expressed during exponential growth in
                     Sauton's minimal media by RNA-sequencing (TPM > 20)."
BEGIN
        1 MIPVLPPLEA LLDRLYVVAL PMRVRFRGIT TREVALIEGP AGWGEFGAFV EYQSAQACAW
       61 LASAIETAYC APPPVRRDRV PINATVPAVA AAQVGEVLAR FPGARTAKVK VAEPGQSLAD
      121 DIERVNAVRE LVPMVRVDAN GGWGVAEAVA AAAALTADGP LEYLEQPCAT VAELAELRRR
      181 VDVPIAADES IRKAEDPLAV VRAQAADIAV LKVAPLGGIS ALLDIAARIA VPVVVSSALD
      241 SAVGIAAGLT AAAALPELDH ACGLGTGGLF EEDVAEPAAP VDGFLAVART TPDPARLQAL
      301 GAPPQRRQWW IDRVKACYSL LVPSFG
//