LOCUS CAB5248276.1 326 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE MUCONATE CYCLOISOMERASE MENC (CIS,CIS-MUCONATE LACTONIZING ENZYME) (MLE) protein. ACCESSION LT708304-575 PROTEIN_ID CAB5248276.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="menC" /locus_tag="BQ2027_MB0568" /note="Mb0568, menC, len: 326 aa. Equivalent to Rv0553, len: 326 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 326 aa overlap). Probable menC, muconate cycloisomerase (EC 5.5.1.1), equivalent to NP_302476.1|NC_002677 putative isomerase/racemase from Mycobacterium leprae (334 aa). Also similar to other muconate cycloisomerases e.g. TCBD_PSESP|P27099 chloromuconate cycloisomerase (370 aa), FASTA scores: opt: 249, E(): 7.8e-09, (32.7% identity in 199 aa overlap). Also similar to O-succinylbenzoate-CoA synthases. BELONGS TO THE MANDELATE RACEMASE / MUCONATE LACTONIZING ENZYME FAMILY. Protein product from Mb0568 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0568 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." BEGIN 1 MIPVLPPLEA LLDRLYVVAL PMRVRFRGIT TREVALIEGP AGWGEFGAFV EYQSAQACAW 61 LASAIETAYC APPPVRRDRV PINATVPAVA AAQVGEVLAR FPGARTAKVK VAEPGQSLAD 121 DIERVNAVRE LVPMVRVDAN GGWGVAEAVA AAAALTADGP LEYLEQPCAT VAELAELRRR 181 VDVPIAADES IRKAEDPLAV VRAQAADIAV LKVAPLGGIS ALLDIAARIA VPVVVSSALD 241 SAVGIAAGLT AAAALPELDH ACGLGTGGLF EEDVAEPAAP VDGFLAVART TPDPARLQAL 301 GAPPQRRQWW IDRVKACYSL LVPSFG //