LOCUS CAB5248270.1 314 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 naphthoate synthase menb (dihydroxynaphthoic acid synthetase) (dhna synthetase) protein. ACCESSION LT708304-569 PROTEIN_ID CAB5248270.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="menB" /locus_tag="BQ2027_MB0562C" /note="Mb0562c, menB, len: 314 aa. Equivalent to Rv0548c, len: 314 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 314 aa overlap). Probable menB, naphthoate synthase (dihydroxynaphthonic acid synthase) (EC 4.1.3.36), equivalent to NP_302473.1|NC_002677 naphthoate synthase from Mycobacterium leprae (300 aa). Also similar to others e.g. MENB_ECOLI|P27290 naphthoate synthase from Escherichia coli (285 aa), FASTA scores: opt: 599, E(): 9.3e-33, (48.1 identity in 285 aa overlap); etc. BELONGS TO THE ENOYL-COA HYDRATASE/ISOMERASE FAMILY. Protein product from Mb0562c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0562c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MVAPAGEQGR SSTALSDNPF DAKAWRLVDG FDDLTDITYH RHVDDATVRV AFNRPEVRNA 61 FRPHTVDELY RVLDHARMSP DVGVVLLTGN GPSPKDGGWA FCSGGDQRIR GRSGYQYASG 121 DTADTVDVAR AGRLHILEVQ RLIRFMPKVV ICLVNGWAAG GGHSLHVVCD LTLASREYAR 181 FKQTDADVGS FDGGYGSAYL ARQVGQKFAR EIFFLGRTYT AEQMHQMGAV NAVAEHAELE 241 TVGLQWAAEI NAKSPQAQRM LKFAFNLLDD GLVGQQLFAG EATRLAYMTD EAVEGRDAFL 301 QKRPPDWSPF PRYF //