LOCUS CAB5248264.1 362 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE O-SUCCINYLBENZOIC ACID--COA LIGASE MENE (OSB-COA SYNTHETASE) (O-SUCCINYLBENZOATE-COA SYNTHASE) protein. ACCESSION LT708304-563 PROTEIN_ID CAB5248264.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="menE" /locus_tag="BQ2027_MB0556C" /note="Mb0556c, menE, len: 362 aa. Equivalent to Rv0542c, len: 362 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 362 aa overlap). Possible menE, O-succinylbenzoic acid-CoA ligase (EC 6.2.1.26), highly similar to Q50170|AAA63145.1|U15187|XCLB 4-Coumarate--CoA ligase from Mycobacterium leprae (352 aa), FASTA scores: opt: 1815, E(): 0, (78.9% identity in 351 aa overlap). Also similar to N-terminus of acid-CoA ligases e.g. NP_471116.1|NC_003212 O-succinylbenzoic acid-CoA ligase from Listeria innocua (469 aa); NP_390957.1|NC_000964 O-succinylbenzoic acid-CoA ligase from Bacillus subtilis (486 aa); MENE_HAEIN|P44565 O-succinylbenzoic acid-CoA ligase from Haemophilus influenzae (452 aa), FASTA scores: opt: 307, E(): 4.6e-12, (25.4% identity in 339 aa overlap); etc. Also some similarity with fadD proteins from Mycobacterium tuberculosis. Contains PS00455 Putative AMP-binding domain signature. BELONGS TO THE ATP-DEPENDENT AMP-BINDING ENZYME FAMILY. Protein product from Mb0556c detected using SWATH mass spectrometry. Mb0556c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MLGGSDPALV AVPTQHESLL GALRVGEQID DDVALVVTTS GTTGPPKGAM LTAAALTASA 61 SAAHDRLGGP GSWLLAVPPY HIAGLAVLVR SVIAGSVPVE LNVSAGFDVT ELPNAIKRLG 121 SGRRYTSLVA AQLAKALTDP AATAALAELD AVLIGGGPAP RPILDAAAAA GITVVRTYGM 181 SETSGGCVYD GVPLDGVRLR VLAGGRIAIG GATLAKGYRN PVSPDPFAEP GWFHTDDLGA 241 LESGDSGVLT VLGRADEAIS TGGFTVLPQP VEAALGTHPA VRDCAVFGLA DDRLGQRVVA 301 AIVVGDGCPP PTLEALRAHV ARTLDVTAAP RELHVVNVLP RRGIGKVDRA ALVRRFAGEA 361 DQ //