LOCUS       CAB5248264.1             362 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            O-SUCCINYLBENZOIC ACID--COA LIGASE MENE (OSB-COA SYNTHETASE)
            (O-SUCCINYLBENZOATE-COA SYNTHASE) protein.
ACCESSION   LT708304-563
PROTEIN_ID  CAB5248264.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="menE"
                     /locus_tag="BQ2027_MB0556C"
                     /note="Mb0556c, menE, len: 362 aa. Equivalent to Rv0542c,
                     len: 362 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 362 aa overlap). Possible menE,
                     O-succinylbenzoic acid-CoA ligase (EC 6.2.1.26), highly
                     similar to Q50170|AAA63145.1|U15187|XCLB 4-Coumarate--CoA
                     ligase from Mycobacterium leprae (352 aa), FASTA scores:
                     opt: 1815, E(): 0, (78.9% identity in 351 aa overlap).
                     Also similar to N-terminus of acid-CoA ligases e.g.
                     NP_471116.1|NC_003212 O-succinylbenzoic acid-CoA ligase
                     from Listeria innocua (469 aa); NP_390957.1|NC_000964
                     O-succinylbenzoic acid-CoA ligase from Bacillus subtilis
                     (486 aa); MENE_HAEIN|P44565 O-succinylbenzoic acid-CoA
                     ligase from Haemophilus influenzae (452 aa), FASTA scores:
                     opt: 307, E(): 4.6e-12, (25.4% identity in 339 aa
                     overlap); etc. Also some similarity with fadD proteins
                     from Mycobacterium tuberculosis. Contains PS00455 Putative
                     AMP-binding domain signature. BELONGS TO THE ATP-DEPENDENT
                     AMP-BINDING ENZYME FAMILY. Protein product from Mb0556c
                     detected using SWATH mass spectrometry. Mb0556c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
BEGIN
        1 MLGGSDPALV AVPTQHESLL GALRVGEQID DDVALVVTTS GTTGPPKGAM LTAAALTASA
       61 SAAHDRLGGP GSWLLAVPPY HIAGLAVLVR SVIAGSVPVE LNVSAGFDVT ELPNAIKRLG
      121 SGRRYTSLVA AQLAKALTDP AATAALAELD AVLIGGGPAP RPILDAAAAA GITVVRTYGM
      181 SETSGGCVYD GVPLDGVRLR VLAGGRIAIG GATLAKGYRN PVSPDPFAEP GWFHTDDLGA
      241 LESGDSGVLT VLGRADEAIS TGGFTVLPQP VEAALGTHPA VRDCAVFGLA DDRLGQRVVA
      301 AIVVGDGCPP PTLEALRAHV ARTLDVTAAP RELHVVNVLP RRGIGKVDRA ALVRRFAGEA
      361 DQ
//