LOCUS CAB5248261.1 210 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE DOLICHYL-PHOSPHATE SUGAR SYNTHASE (DOLICHOL-PHOSPHATE SUGAR SYNTHETASE) (DOLICHOL-PHOSPHATE SUGAR TRANSFERASE) (SUGAR PHOSPHORYLDOLICHOL SYNTHASE) protein. ACCESSION LT708304-560 PROTEIN_ID CAB5248261.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0553" /note="Mb0553, -, len: 210 aa. Equivalent to Rv0539, len: 210 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 210 aa overlap). Probable dolichol-P-sugar synthase (EC 2.4.1.-), highly similar to CAB76989.1|AL159178 putative glycosyltransferase from Streptomyces coelicolor (242 aa), and similar to various dolichol-P-sugar synthetases and sugar transferases e.g. NP_126257.1|NC_000868 DOLICHYL-PHOSPHATE MANNOSE SYNTHASE RELATED PROTEIN from Pyrococcus abyssi (211 aa); N-terminus of NP_127133.1|NC_000868 DOLICHOL-P-GLUCOSE SYNTHETASE from Pyrococcus abyssi (378 aa); N-terminus of NP_068880.1|NC_000917 putative dolichol-P-glucose synthetase from Archaeoglobus fulgidus (369 aa), FASTA scores: E(): 2.4e-13, (32. 1% identity in 193 aa overlap); Q26732 DOLICHYL-PHOSPHATE-MANNOSE SYNTHASE PRECURSOR from TRYPANOSOMA BRUCEI (267 aa), FASTA scores: opt: 179, E(): 0.0011, (30.7% identity in 205 aa overlap); etc. Also similar to Rv2051c|MTY25D10_18 from Mycobacterium tuberculosis. Contains S00017 ATP/GTP-binding site motif A (P-loop). Protein product from Mb0553 detected using SWATH mass spectrometry. Mb0553 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MLPCLNEEES LPAVLAAIPA GYRALVVDNN STDDTATVAA RHGAQVVVEP RPGYGSAVHA 61 GVLAATTPIV AVIDADGSMD AGDLPKLVAE LDKGADLVTG RRRPVAGLHW PWVARVGTVV 121 MSWRLRTRHR LPVHDIAPMR VARREALLDL GVVDRRSGYP LELLVRAAAA GWRVVELDVS 181 YGPRTGGKSK VSGSLRGSII AILDFWKVIS //