LOCUS       CAB5248258.1             346 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            UDP-GLUCOSE 4-EPIMERASE GALE3 (GALACTOWALDENASE) (UDP-
            GALACTOSE 4-EPIMERASE) (URIDINE DIPHOSPHATE GALACTOSE
            4-EPIMERASE) (URIDINE DIPHOSPHO-GALACTOSE 4-EPIMERASE)
            protein.
ACCESSION   LT708304-557
PROTEIN_ID  CAB5248258.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="galE3"
                     /locus_tag="BQ2027_MB0550"
                     /note="Mb0550, galE3, len: 346 aa. Equivalent to Rv0536,
                     len: 346 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 346 aa overlap). Possible galE3,
                     UDP-glucose 4-epimerase (EC 5.1.3.2), highly similar to
                     CAB76986.1|AL159178 putative epimerase from Streptomyces
                     coelicolor (334 aa); and similar to other epimerases e.g.
                     NP_436775.1|NC_003078 putative NDP-glucose
                     dehydrataseepimerase protein from Sinorhizobium meliloti
                     (368 aa); AF143772|AF143772_7 GepiA from Mycobacterium
                     avium strain 2151 (353 aa), FASTA scores: opt: 577, E():
                     3.9e-29, (36.6% identity in 352 aa overlap);
                     GALE_METJA|Q57664 putative UDP-glucose 4-epimerase (305
                     aa), FASTA scores: opt: 300, E(): 1.6e-12, (30.9% identity
                     in 343 aa overlap); etc. Also similar to Mycobacterium
                     tuberculosis proteins e.g. Rv3634c, Rv3784, etc. SEEMS TO
                     BELONG TO THE SUGAR EPIMERASE FAMILY. Note that previously
                     known as galE2. Protein product from Mb0550 detected using
                     SWATH mass spectrometry. Mb0550 found to be expressed
                     during exponential growth in Sauton's minimal media by
                     RNA-sequencing."
BEGIN
        1 MRVLLTGAAG FIGSRVDAAL RAAGHDVVGV DALLPAAHGP NPVLPPGCQR VDVRDASALA
       61 PLLAGVDLVC HQAAMVGAGV NAADAPAYGG HNDFATTVLL AQMFAAGVRR LVLASSMVVY
      121 GQGRYDCPQH GPVDPLPRRR ADLDNGVFEH RCPGCGEPVI WQLVDEDAPL RPRSLYAASK
      181 TAQEHYALAW SEASGGSVVA LRYHNVYGPG MPRDTPYSGV AAIFRSAVEK GKPPKVFEDG
      241 GQMRDFVHVD DVAAANLAAV HLGEADRDGF TAVNVCSGRP ISILQVATAI CDARGGSMSP
      301 AITGHYRSGD VRHIVADPAR AARVLGFRAA VDPGEGLREF AFAPLR
//