LOCUS CAB5248258.1 346 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE UDP-GLUCOSE 4-EPIMERASE GALE3 (GALACTOWALDENASE) (UDP- GALACTOSE 4-EPIMERASE) (URIDINE DIPHOSPHATE GALACTOSE 4-EPIMERASE) (URIDINE DIPHOSPHO-GALACTOSE 4-EPIMERASE) protein. ACCESSION LT708304-557 PROTEIN_ID CAB5248258.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="galE3" /locus_tag="BQ2027_MB0550" /note="Mb0550, galE3, len: 346 aa. Equivalent to Rv0536, len: 346 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 346 aa overlap). Possible galE3, UDP-glucose 4-epimerase (EC 5.1.3.2), highly similar to CAB76986.1|AL159178 putative epimerase from Streptomyces coelicolor (334 aa); and similar to other epimerases e.g. NP_436775.1|NC_003078 putative NDP-glucose dehydrataseepimerase protein from Sinorhizobium meliloti (368 aa); AF143772|AF143772_7 GepiA from Mycobacterium avium strain 2151 (353 aa), FASTA scores: opt: 577, E(): 3.9e-29, (36.6% identity in 352 aa overlap); GALE_METJA|Q57664 putative UDP-glucose 4-epimerase (305 aa), FASTA scores: opt: 300, E(): 1.6e-12, (30.9% identity in 343 aa overlap); etc. Also similar to Mycobacterium tuberculosis proteins e.g. Rv3634c, Rv3784, etc. SEEMS TO BELONG TO THE SUGAR EPIMERASE FAMILY. Note that previously known as galE2. Protein product from Mb0550 detected using SWATH mass spectrometry. Mb0550 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MRVLLTGAAG FIGSRVDAAL RAAGHDVVGV DALLPAAHGP NPVLPPGCQR VDVRDASALA 61 PLLAGVDLVC HQAAMVGAGV NAADAPAYGG HNDFATTVLL AQMFAAGVRR LVLASSMVVY 121 GQGRYDCPQH GPVDPLPRRR ADLDNGVFEH RCPGCGEPVI WQLVDEDAPL RPRSLYAASK 181 TAQEHYALAW SEASGGSVVA LRYHNVYGPG MPRDTPYSGV AAIFRSAVEK GKPPKVFEDG 241 GQMRDFVHVD DVAAANLAAV HLGEADRDGF TAVNVCSGRP ISILQVATAI CDARGGSMSP 301 AITGHYRSGD VRHIVADPAR AARVLGFRAA VDPGEGLREF AFAPLR //