LOCUS CAB5248248.1 529 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CONSERVED TRANSMEMBRANE PROTEIN protein. ACCESSION LT708304-547 PROTEIN_ID CAB5248248.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0541" /note="Mb0541, -, len: 529 aa. Equivalent to Rv0528, len: 529 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 529 aa overlap). Probable conserved transmembrane protein, equivalent (shorter 14 aa in N-terminus) to CAC31926.1|AL583925 conserved membrane protein from Mycobacterium leprae (542 aa). Also highly similar to Q49817|B2168_C2_237|S72902 hypothetical protein from Mycobacterium leprae (364 aa), FASTA scores: opt: 1846, E(): 0, (81.1% identity in 338 aa overlap); and Q49811|B2168_C1_194|S72891 hypothetical protein from Mycobacterium leprae (106 aa), FASTA scores: opt: 506, E(): 3.8e-26, (73.6% identity in 106 aa overlap). Also highly similar to CAC08381.1|AL392176 putative integral membrane protein from Streptomyces coelicolor (574 aa). Protein product from Mb0541 detected using SWATH mass spectrometry. Mb0541 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MWRSLTSMGT ALVLLFLLAL AAIPGALLPQ RGLNAAKVDD YLAAHPLIGP WLDELQAFDV 61 FSSFWFTAIY VLLFVSLVGC LAPRTIEHAR SLRATPVAAP RNLARLPKHA HARLAGEPAA 121 LAATITGRLR GWRSITRQQG DSVEVSAEKG YLREFGNLVF HFALLGLLVA VAVGKLFGYE 181 GNVIVIADGG PGFCSASPAA FDSFRAGNTV DGTSLHPICV RVNNFQAHYL PSGQATSFAA 241 DIDYQADPAT ADLIANSWRP YRLQVNHPLR VGGDRVYLQG HGYAPTFTVT FPDGQTRTST 301 VQWRPDNPQT LLSAGVVRID PPAGSYPNPD ERRKHQIAIQ GLLAPTEQLD GTLLSSRFPA 361 LNAPAVAIDI YRGDTGLDSG RPQSLFTLDH RLIEQGRLVK EKRVNLRAGQ QVRIDQGPAA 421 GTVVRFDGAV PFVNLQVSHD PGQSWVLVFA ITMMAGLLVS LLVRRRRVWA RITPTTAGTV 481 NVELGGLTRT DNSGWGAEFE RLTGRLLAGF EARSPDMAEA AAGTGRDVD //