LOCUS       CAB5248221.1             376 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE
            UDP-GLUCOSE 4-EPIMERASE GALE2 (GALACTOWALDENASE) (UDP-
            GALACTOSE 4-EPIMERASE) (URIDINE DIPHOSPHATE GALACTOSE
            4-EPIMERASE) (URIDINE DIPHOSPHO-GALACTOSE 4-EPIMERASE)
            protein.
ACCESSION   LT708304-520
PROTEIN_ID  CAB5248221.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="galE2"
                     /locus_tag="BQ2027_MB0513"
                     /note="Mb0513, galE2, len: 376 aa. Equivalent to Rv0501,
                     len: 376 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 376 aa overlap). Possible galE2,
                     UDP-glucose 4-epimerase (EC 5.1.3.2), highly similar
                     (except in N-terminus) to CAC31944.1|AL583925 possible
                     glucose epimerase/dehydratase from Mycobacterium leprae
                     (364 aa). N-terminus highly similar to
                     S72923|B2168_C1_174|467075|AAA17259.1|U00018 hypothetical
                     protein from Mycobacterium leprae (180 aa), FASTA scores:
                     opt: 934, E(): 0, (89.6% identity in 164 aa overlap); and
                     C-terminus highly similar to
                     S72898|467050|AAA17234.1|U00018 hypothetical protein from
                     Mycobacterium leprae (168 aa), FASTA scores: opt: 928,
                     E(): 0, (82.7% identity in 168 aa overlap). Also highly
                     similar to T36274|5123671|CAB45360.1|AL079345 probable
                     epimerase from Streptomyces coelicolor (353 aa); and
                     similar in part to other epimerases e.g. GALE_ECOLI|P09147
                     UDP-glucose 4-epimerase from Escherichia coli (338 aa),
                     FASTA scores: opt: 241, E(): 6.7e-09, (28.2% identity in
                     294 aa overlap); etc. BELONGS TO THE SUGAR EPIMERASE
                     FAMILY. COFACTOR: NAD. Note that previously known as
                     galE1. Protein product from Mb0513 detected using SWATH
                     mass spectrometry. Mb0513 found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
BEGIN
        1 MSSSNGRGGA GGVGGSSEHP QYPKVVLVTG ACRFLGGYLT ARLAQNPLIN RVIAVDAIAP
       61 SKDMLRRMGR AEFVRADIRN PFIAKVIRNG EVDTVVHAAA ASYAPRSGGS AALKELNVMG
      121 AMQLFAACQK APSVRRVVLK STSEVYGSSP HDPVMFTEDS SSRRPFSQGF PKDSLDIEGY
      181 VRALGRRRPD IAVTILRLAN MIGPAMDTTL SRYLAGPLVP TIFGRDARLQ LLHEQDALGA
      241 LERAAMAGKA GTFNIGADGI LMLSQAIRRA GRIPVPVPGF GVWALDSLRR ANHYTELNRE
      301 QFAYLSYGRV MDTTRMRVEL GYQPKWTTVE AFDDYFRGRG LTPIIDPHRV RSWEGRAVGL
      361 AQRWGSRNPI PWSGLR
//