LOCUS CAB5248214.1 328 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Exopolyphosphatas e (EC protein. ACCESSION LT708304-513 PROTEIN_ID CAB5248214.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ppx1" /locus_tag="BQ2027_MB0507" /EC_number="3.6.1.11" /note="Mb0507, -, len: 328 aa. Equivalent to Rv0496, len: 328 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 328 aa overlap). Conserved hypothetical protein, highly similar to S72894|467046|AAA17230.1|U00018 exopolyphosphatase (EC 3.6.1.11) ppx from Mycobacterium leprae (406 aa), FASTA scores: opt: 1902, E(): 0, (86.6% identity in 343 aa overlap); and P54882|Y496_MYCLE|ML2434|13094008|CAC31951.1 |AL583925 HYPOTHETICAL 36.2 KDA PROTEIN from Mycobacterium leprae (339 aa). Also highly similar to hypothetical proteins and exopolyphosphatases e.g. Q9X8H1|Y715_STRCO|SCE7.15c HYPOTHETICAL PROTEIN from Streptomyces coelicolor (309 aa). C-terminal region similar to CGU31224_1|Q46054 protein similar to ppx gene product of Mycobacterium leprae from Cornybacterium glutamicum (140 aa), FASTA scores: opt: 615, E(): 2.7e-33, (70.9% identity in 134 aa overlap). Protein product from Mb0507 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0507 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MVDAHRGGHP TPMSSTKATL RLAEATDSSG KITKRGADKL ISTIDEFAKI AISSGCAELM 61 AFATSAVRDA ENSEDVLSRV RKETGVELQA LRGEDESRLT FLAVRRWYGW SAGRILNLDI 121 GGGSLEVSSG VDEEPEIALS LPLGAGRLTR EWLPDDPPGR RRVAMLRDWL DAELAEPSVT 181 VLEAGSPDLA VATSKTFRSL ARLTGAAPSM AGPRVKRTLT ANGLRQLIAF ISRMTAVDRA 241 ELEGVSADRA PQIVAGALVA EASMRALSIE AVEICPWALR EGLILRKLDS EADGTALIES 301 SSVHTSVRAV GGQPADRNAA NRSRGSKP //