LOCUS CAB5248209.1 629 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE OXIDOREDUCTASE GMC-TYPE protein. ACCESSION LT708304-508 PROTEIN_ID CAB5248209.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0502C" /note="Mb0502c, -, len: 629 aa. Equivalent to Rv0492c, len: 629 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 629 aa overlap). Probable oxidoreductase GMC type (EC 1.-.-.-), similar to others except in N-terminus e.g. P55582|AE000087_5|Y4NJ_RHISN HYPOTHETICAL GMC-TYPE OXIDOREDUCTASE from Rhizobium sp. (505 aa), FASTA scores: opt: 873, E():0, (34.3% identity in 502 aa overlap); YTH2_RHOER|P46371 HYPOTHETICAL 53.0 kd GMC-TYPE OXIDOREDUCTASE from Rhodococcus erythropolis (493 aa), FASTA score: (25.7% identity in 521 aa overlap); YTH2_RHOSO|P46371 hypothetical 53.0 kd gmc-type oxidoreductase from Rhodococcus erythropolis (493 aa), FASTA score: (25.7% identity in 521 aa overlap); NP_085596.1|NC_002679 probable oxidoreductase from Mesorhizobium loti (507 aa); NP_285451.1|NC_001264 GMC oxidoreductase from Deinococcus radiodurans (722 aa); NP_249055.1|NC_002516 probable oxidoreductase from Pseudomonas aeruginosa (531 aa); etc. Contains PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature, and PS00624 GMC oxidoreductases signature 2. BELONGS TO THE GMC OXIDOREDUCTASES FAMILY. COFACTOR: FAD (BY SIMILARITY). Note that start changed since first submission (previously 684 aa). Protein product from Mb0502c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0502c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MSRLADRAKS YPLASFGAAL LPPELGGPLP AQFVQRVDRY VTRLPATSRF AVRAGLASLA 61 AASYLTTGRS LPRLHPDERA RVLHRIAALS PEVAAAVEGL KAIVLLANGA DTYAHELLAR 121 AQEHDAARPD AELTVILSAD SPSVTRADAV VVGSGAGGAM VARTLARAGL DVVVLEEGRR 181 WTVEEFRSTH PVDRYAGLYR GAGATVALGR PAVVLPMGRA VGGTTVVNSG TCFRPSLAVQ 241 RRWRDEFGLG LADPDQLGRR LDDAEQTLRV APVPLEIMGR NGRLLLQAAK SLGWRAAPIP 301 RNAPGCRGCC QCAIGCPSNA KFGVHLNALP QACAAGARII SWARVERILH RAGRAYGVRA 361 RRPDGTTLDV LADAVVVAAG ATETPGLLRR SGLGGHPRLG HNLALHPATM LAGLFDDDVF 421 AWRGVLQSAA VHEFHESDGV LIEATSTPPG MGSMVFPGYG AELLRWLDRA PQIATFGAMV 481 ADRGVGTVRS VRGETVVRYD IAPGEIAKLR VALQAIGRLF FAAGAVEVLT GIPGAPPMRS 541 LPELQDVLRR ANPRSLHLAA FHPTGTAAAG ADEQLCPVDA TGRLRGVEGV WVADASILPS 601 CPEVNPQLSI MAMALAVADQ TVAKVVGVR //