LOCUS CAB5248173.1 676 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE PEPTIDASE protein. ACCESSION LT708304-472 PROTEIN_ID CAB5248173.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0466C" /note="Mb0466c, -, len: 676 aa. Equivalent to Rv0457c, len: 673 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 673 aa overlap). Probable peptidase (EC 3.4.-.-), similar to many e.g. NP_102851.1|14022026|BAB48637.1 probable endopeptidase from Mesorhizobium loti (687 aa); Y4NA_RHISN|P55577 probable peptidase (EC 3.4.21.-) (726 aa), FASTA scores: opt: 1126, E(): 0, (40.9% identity in 491 aa overlap). Also similar to Mycobacterium tuberculosis protein MTCY369.26 FASTA score: (33.8% identity in 299 aa overlap). REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, a possible RBS upstream leads to an earlier start resulting in a slightly longer product compared to its homolog in Mycobacterium tuberculosis strain H37Rv (676 aa versus 673 aa). Protein product from Mb0466c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0466c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MASMTFEPAP DGADPYLWLE DVTGAEALDW VRARNKPTTA AFCDAEFERM RVEALEVLDT 61 DARIPYVNRR GNYLYNFWRD AANPRGLWRR TTLDSYRTDS PGWDVLIDVD ELGRADDQKW 121 VWGGAGVIEP DYTRALIGLS PGGSDASIVR EFDMLTREFV EDGFQLPPAK SQITWEDPDT 181 VLLGTDFGGD SLTTSGYPRV IKRWRRGKPL ADAETIFEGA GTDVRVNASA DRTPGFERTL 241 LGRALDFWNE EVYELRGSEL IRIEAPTDAS VSIHRDWLLI ELRTDWTVAT TRYTAGSLLA 301 AEYDEFLAGS AELQVVFEPD EHTALYQYAW TRDRLLIVTL ADVASRVEIA TPGSWRREPL 361 SGIPAATNTV IVSADSHGDE FFLDSSGFDT PSRLMRGTDD GRLAEIKSAP AFFDAENMAV 421 TQYFATSDDG TSIPYFVVRR TDADNPGPTL LNGYGGFETS RTPTYDGVLG RLWLARGGTY 481 ALANIRGGGE YGPGWHTQAM REGRDKVAQD FAAVATDLVT RGITTAEQLG ARGGSNGGLL 541 MGIMLTGYPE KFGALVCDVP LLDMKRYHLL LAGASWMAEY GDPDNPDDWK FISEYSPYQN 601 ISANRKYPPV LMTTSTRDDR VHPGHARKMT AALQAAGHPV WYYENIEGGH AGAADNAQIA 661 FKSALSFAFL WRMLAG //