LOCUS       CAB5248170.1             304 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 ENOYL-
            COA HYDRATASE ECHA2 (ENOYL HYDRASE) (UNSATURATED ACYL-
            COA HYDRATASE) (CROTONASE) protein.
ACCESSION   LT708304-469
PROTEIN_ID  CAB5248170.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="echA2"
                     /locus_tag="BQ2027_MB0464C"
                     /note="Mb0464c, echA2, len: 304 aa. Equivalent to Rv0456c,
                     len 304 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 304 aa overlap). Probable echA2,
                     enoyl-CoA hydratase (EC 4.2.1.17), similar to other
                     enoyl-coA hydratases e.g. Q13011 PEROXISOMAL ENOYL-COA
                     HYDRATASE-LIKE PROTEIN (328 aa), FASTA scores: opt: 209,
                     E(): 5.3e-07, (31.7% identity in 142 aa overlap). Also
                     similar to several other proteins from Mycobacterium
                     tuberculosis e.g. MTCY09F9.29 FASTA score: (32.9% identity
                     in 146 aa overlap); and MTI376.01c. Protein product from
                     Mb0464c detected using shotgun mass spectrometry and SWATH
                     mass spectrometry. Mb0464c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
BEGIN
        1 MPTPDFQTLL YTTAGPVATI TLNRPEQLNT IVPPMPDEIE AAIGLAERDQ DIKVIVLRGA
       61 GRAFSGGYDF GGGFQHWGDA MMTDGRWDPG KDFAMVTARE TGPTQKFMAI WRASKPVIAQ
      121 VHGWCVGGAS DYALCADIVI ASEDAVIGTP YSRMWGAYLT GMWLYRLSLA KVKWHSLTGR
      181 PLTGVQAAEA ELINEAVPFE RLEARVAEIA TELARIPLSQ LQAQKLIVNQ AYENMGLAST
      241 QLLGGILDGL MRNTPDALEF IRTAQTQGVR AAVERRDGPF GDYSQAPPEL RPDPTHVITP
      301 DGSM
//