LOCUS CAB5248170.1 304 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 ENOYL- COA HYDRATASE ECHA2 (ENOYL HYDRASE) (UNSATURATED ACYL- COA HYDRATASE) (CROTONASE) protein. ACCESSION LT708304-469 PROTEIN_ID CAB5248170.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="echA2" /locus_tag="BQ2027_MB0464C" /note="Mb0464c, echA2, len: 304 aa. Equivalent to Rv0456c, len 304 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 304 aa overlap). Probable echA2, enoyl-CoA hydratase (EC 4.2.1.17), similar to other enoyl-coA hydratases e.g. Q13011 PEROXISOMAL ENOYL-COA HYDRATASE-LIKE PROTEIN (328 aa), FASTA scores: opt: 209, E(): 5.3e-07, (31.7% identity in 142 aa overlap). Also similar to several other proteins from Mycobacterium tuberculosis e.g. MTCY09F9.29 FASTA score: (32.9% identity in 146 aa overlap); and MTI376.01c. Protein product from Mb0464c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0464c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MPTPDFQTLL YTTAGPVATI TLNRPEQLNT IVPPMPDEIE AAIGLAERDQ DIKVIVLRGA 61 GRAFSGGYDF GGGFQHWGDA MMTDGRWDPG KDFAMVTARE TGPTQKFMAI WRASKPVIAQ 121 VHGWCVGGAS DYALCADIVI ASEDAVIGTP YSRMWGAYLT GMWLYRLSLA KVKWHSLTGR 181 PLTGVQAAEA ELINEAVPFE RLEARVAEIA TELARIPLSQ LQAQKLIVNQ AYENMGLAST 241 QLLGGILDGL MRNTPDALEF IRTAQTQGVR AAVERRDGPF GDYSQAPPEL RPDPTHVITP 301 DGSM //