LOCUS CAB5248166.1 236 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE TRANSCRIPTIONAL REGULATORY PROTEIN protein. ACCESSION LT708304-465 PROTEIN_ID CAB5248166.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0460" /note="Mb0460, -, len: 236 aa. Equivalent to Rv0452, len: 236 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 236 aa overlap). Possible transcriptional regulator, similar to several putative TetR-family transcriptional regulators from Streptomyces coelicolor. Also similar in N-terminus to U1740Y|U15183|MLU15183_33 from Mycobacterium leprae (67 aa), FASTA score: (76.1% identity in 67 aa overlap). Contains probable helix-turn-helix motif at aa 44-65 (Score 1727, +5.07 SD). Protein product from Mb0460 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0460 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MRYPLAVAQL GFQRARTEEN KRQRAAALVE AARSLALETG VASVTLTAVA GRAGIHYSAV 61 RRYFTSHKEV LLHLAAEGWA RWSGTVCEQL GEPGPMSAPR VAEALANGLA ADPLFCDLLA 121 NLHLHLEQEV DVDRVIEVKR TSIAAVIALV DAIESALPAL GRSGAFDILL AAYSLAATLW 181 QIANPPERLT DAYAEEPELL PPEWNLDFAA ALTRLLTATL LGLLAGSPCE CRSPTR //