LOCUS CAB5248123.1 385 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ACETATE KINASE ACKA (ACETOKINASE) protein. ACCESSION LT708304-422 PROTEIN_ID CAB5248123.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="ackA" /locus_tag="BQ2027_MB0417" /note="Mb0417, ackA, len: 385 aa. Equivalent to Rv0409, len: 385 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 385 aa overlap). Probable ackA, acetate kinase (EC 2.7.2.1), highly similar to others e.g. ACKA_BACSU|P37877 acetate kinase from Bacillus subtilis (395 aa), FASTA scores: opt: 974, E(): 0, (43.5% identity in 393 aa overlap); etc. Contains PS01075 Acetate and butyrate kinases family signature 1, PS00758 ArgE / dapE / ACY1/ CPG2 / yscS family signature 1. BELONGS TO THE ACETOKINASE FAMILY. Protein product from Mb0417 detected using SWATH mass spectrometry. Mb0417 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MSSTVLVINS GSSSLKFQLV EPVAGMSRAA GIVERIGERS SPVADHAQAL HRAFKMLAED 61 GIDLQTCGLV AVGHRVVHGG TEFHQPTLLD DTVIGKLEEL SALAPLHNPP AVLGIKVARR 121 LLANVAHVAV FDTAFFHDLP PAAATYAIDR DVADRWHIRR YGFHGTSHQY VSERAAAFLG 181 RPLDGLNQIV LHLGNGASAS AIARGRPVET SMGLTPLEGL VMGTRSGDLD PGVISYLWRT 241 ARMGVEDIES MLNHRSGMLG LAGERDFRRL RLVIETGDRS AQLAYEVFIH RLRKYLGAYL 301 AVLGHTDVVS FTAGIGENDA AVRRDALAGL QGLGIALDQD RNLGPGHGAR RISSDDSPIA 361 VLVVPTNEEL AIARDCLRVL GGRRA //