LOCUS CAB5248120.1 272 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 BETA LACTAMASE LIKE PROTEIN protein. ACCESSION LT708304-419 PROTEIN_ID CAB5248120.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0414C" /note="Mb0414c, -, len: 272 aa. Equivalent to Rv0406c, len: 272 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 272 aa overlap). Beta-lactamase-like protein, equivalent to AAD38170.1|AF152397_1 beta-lactamase-like protein from Mycobacterium phlei (243 aa); AL023514|MLCB4_8 hypothetical protein from Mycobacterium leprae (251 aa), FASTA scores: opt: 1284, E(): 0, (74.9% identity in 243 aa overlap); and AAD38164.1|AF152394_2 beta-lactamase-like protein from Mycobacterium avium (247 aa), FASTA scores: opt: 1301, E(): 0, (74.2% identity in 244 aa overlap); etc. Also slight similarity to others beta-lactamases and hypothetical proteins e.g. P52700|BLA1_XANMA|628530|S45349 METALLO-BETA-LACTAMASE L1 PRECURSOR (BETA-LACTAMASE, TYPE II) (PENICILLINASE) from Xanthomonas maltophilia (290 aa), FASTA scores: (34.4% identity in 96 aa overlap). Protein product from Mb0414c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0414c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." BEGIN 1 MVATRGTRLA ALALAPRLAG MAELVQITDK VHLARGHAVN WVLVTDDTGV LLIDAGYPGD 61 RAEVLASLNK LGYTPGDVRA IVLTHAHIDH LGSAIWFARE HSTPVYCHAE EVGHAKREYR 121 ENASVFDVAL RSWRPRVAVW GIHLLRRGGL TGDGIPTAQP LTAEAAAGLP GQPMAIFTPG 181 HTSGHCSYVV DGVLASGDAL ITGHPMLRHR GPQLLPAVFS HSQQNSIRSL AALALLETNI 241 LAPGHGELWH GPIRKATDEA LERAQKSNHV FR //