LOCUS CAB5248102.1 406 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE O-SUCCINYLHOMOSERINE SULFHYDRYLASE METZ (OSH SULFHYDRYLASE) protein. ACCESSION LT708304-401 PROTEIN_ID CAB5248102.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="metZ" /locus_tag="BQ2027_MB0397" /note="Mb0397, metZ, len: 406 aa. Equivalent to Rv0391, len: 406 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 406 aa overlap). Probable metZ, O-succinylhomoserine sulfhydrylase (EC 4.2.99.-), equivalent, but shorter 20 aa in N-terminus, to AA18941.1|AL023514 O-succinylhomoserine sulfhydrylase from Mycobacterium leprae (426 aa). Also highly similar to others e.g. METZ_PSEAE|P55218 o-succinylhomoserine sulfhydrylase from Pseudomonas aeruginosa (403 aa), FASTA scores: opt: 1175, E(): 0, (47.2% identity in 392 aa overlap); etc. BELONGS TO THE TRANS-SULFURATION ENZYMES FAMILY. Could also be a cystathionine gamma-synthase (EC 4.2.99.9). Protein product from Mb0397 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0397 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MTDESSVRTP KALPDGVSQA TVGVRGGMLR SGFEETAEAM YLTSGYVYGS AAVAEKSFAG 61 ELDHYVYSRY GNPTVSVFEE RLRLIEGAPA AFATASGMAA VFTSLGALLG AGDRLVAARS 121 LFGSCFVVCS EILPRWGVQT VFVDGDDLSQ WERALSVPTQ AVFFETPSNP MQSLVDIAAV 181 TELAHAAGAK VVLDNVFATP LLQQGFPLGV DVVVYSGTKH IDGQGRVLGG AILGDREYID 241 GPVQKLMRHT GPAMSAFNAW VLLKGLETLA IRVQHSNASA QRIAEFLNGH PSVRWVRYPY 301 LPSHPQYDLA KRQMSGGGTV VTFALDCPED VAKQRAFEVL DKMRLIDISN NLGDAKSLVT 361 HPATTTHRAM GPEGRAAIGL GDGVVRISVG LEDTDDLIAD IDRALS //