LOCUS       CAB5248094.1             179 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            OROTATE PHOSPHORIBOSYLTRANSFERASE PYRE (OPRT) (OPRTASE)
            protein.
ACCESSION   LT708304-393
PROTEIN_ID  CAB5248094.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="pyrE"
                     /locus_tag="BQ2027_MB0389C"
                     /note="Mb0389c, pyrE, len: 179 aa. Equivalent to Rv0382c,
                     len: 179 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 179 aa overlap). Probable pyrE,
                     orotate phosphoribosyltransferase (EC 2.4.2.10),
                     equivalent to CAC32004.1|AL583925 probable
                     purine/pyrimidine phosphoribosyltransferase from
                     Mycobacterium leprae (179 aa). Also highly similar to many
                     others e.g. T36540|4753874|CAB42037.1|AL049754|SCH10.28c
                     probable orotate phosphoribosyltransferase from
                     Streptomyces coelicolor (182 aa);
                     H69115|2622996|AAB86326.1|AE000938_10|MTH1860 probable
                     orotate phosphoribosyltransferase from Methanobacterium
                     thermoautotrophicum (180 aa), FASTA scores: opt: 389, E():
                     2.7e-20, (40.7% identity in 172 aa overlap);
                     O08359|PYRE_SULAC|2065444|CAA73352.1|Y12822 OROTATE
                     PHOSPHORIBOSYLTRANSFERASE from Sulfolobus acidocaldarius
                     (197 aa); etc. Note that also similar to other puridine
                     5'-monophosphate synthases (umpA genes; UMP synthases),
                     generally in N-terminus that corresponds to orotate
                     phosphoribosyltransferase activity. Contains PS00589 PTS
                     HPR component serine phosphorylation site signature.
                     BELONGS TO THE PURINE/PYRIMIDINE PHOSPHORIBOSYLTRANSFERASE
                     FAMILY. Note that previously known as umpA. Protein
                     product from Mb0389c detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb0389c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing (TPM > 20)."
BEGIN
        1 MAGPDRAELA ELVRRLSVVH GRVTLSSGRE ADYYVDLRRA TLHHRASALI GRLMRELTAD
       61 WDYSVVGGLT LGADPVATAI MHAPGRPIDA FVVRKSAKAH GMQRLIEGSE VTGQRVLVVE
      121 DTSTTGNSAL TAVHAVQDVG GEVVGVATVV DRATGAAEAI EAEGLRYRSV LGLADLGLD
//