LOCUS CAB5248067.1 3507 aa PRT BCT 25-MAY-2020
DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 ppe
family protein ppe7 protein.
ACCESSION LT708304-366
PROTEIN_ID CAB5248067.1
SOURCE Mycobacterium tuberculosis variant bovis AF2122/97
ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE 1
AUTHORS Malone K.M.
JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine, University College
Dublin, D4, Ireland
REFERENCE 2
AUTHORS Malone M K., Farrell D., Malone K.
JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary
Medicine, Tuberculosis Molecular Microbiology Research Group,
University College Dublin, Tuberculosis Molecular Microbiology
Research Group, School of Veterinary Medicine,, University College
Dublin, D4, Ireland
FEATURES Qualifiers
source /organism="Mycobacterium tuberculosis variant bovis
AF2122/97"
/chromosome="Mycobacterium_bovis_AF212297"
/isolate="AF2122/97"
/mol_type="genomic DNA"
/isolation_source="Mycobacterium bovis subsp. bovis strain
AF2122/97. This strain is a fully virulent strain that was
isolated in 1997 in the UK from a cow suffering necrotic
lesions in lung and bronchomediastinal lymph nodes. The
strain was also reported to infect and persist in badgers
that are considered to be a significant source of bovine
infection."
/db_xref="taxon:233413"
protein /transl_table=11
/gene="PPE8"
/locus_tag="BQ2027_MB0362C"
/note="Mb0362c, PPE8, len: 3507 aa. Equivalent to Rv0355c
and Rv0354c, len: 3300 aa and 141 aa, from Mycobacterium
tuberculosis strain H37Rv, (99.8% identity in 3296 aa
overlap and 100.0% identity in 125 aa overlap). PPE8,
member of the Mycobacterium tuberculosis PPE family,
similar to others e.g. AL009198|MTV004_5 from M.
tuberculosis (3716 aa), FASTA scores: opt: 2906, E(): 0,
(40.9% identity in 3833 aa overlap); MTV004_3 FASTA
scores: (39.0% identity in 3531 aa overlap); etc. Gene
contains large number of clustered Major Polymorphic
Tandem Repeats (MPTR). Related to MTCY13E10.16c, E(): 0;
MTCY13E10.17c, E(): 0; MTCY48.17, E(): 0; MTCY98.0034c,
E(): 0; MTCY03C7.23 E(): 0; MTCY98.0031c, E(): 0;
MTCY31.06c, E(): 5.6e-17; MTCY359.33, E(): 2.3e-16. PPE7,
member of the Mycobacterium tuberculosis PPE family,
similar to others e.g. MTCY63_9 from Mycobacterium
tuberculosis (2411 aa), FASTA scores: E(): 3.6e-11, (47.6%
identity in 103 aa overlap). Possible continuation of ORF
upstream, but no sequence error apparent.
REMARK-M.bovis-M.tuberculosis: In Mycobacterium
tuberculosis strain H37Rv, PPE7 and PPE8 exist as 2 genes.
In Mycobacterium bovis, a 2 bp insertion (*-ta) resulting
in the absence of a stop codon between the 2 genes, leads
to a single product. Mb0362c found to be expressed during
exponential growth in Sauton's minimal media by
RNA-sequencing."
BEGIN
1 MSFAVLPPEI NSARLYVGAG LAPMLDAAAA WDGLADELGS AAASFSAVTA GLAGSSWLGA
61 ASTAMTGAAA PYLGWLSAAA AQAQQAATQT RLAAAAFEAA LAATVHPAII SANRALFVSL
121 VVSNLLGQNA PAIAATEAAY EQMWAQDVAA MFGYHAGASA AVSALTPFGQ ALPTVAGGGA
181 LVSAAAAQVT TRVFRNLGLA NVGEGNVGNG NVGNFNLGSA NIGNGNIGSG NIGSSNIGFG
241 NVGPGLTAAL NNIGFGNTGS NNIGFGNTGS NNIGFGNTGD GNRGIGLTGS GLLGFGGLNS
301 GTGNIGLFNS GTGNVGIGNS GTGNWGIGNS GNSYNTGFGN SGDANTGFFN SGIANTGVGN
361 AGNYNTGSYN PGNSNTGGFN MGQYNTGYLN SGNYNTGLAN SGNVNTGAFI TGNFNNGFLW
421 RGDHQGLIFG SPGFFNSTSA PSSGFFNSGA GSASGFLNSG ANNSGFFNSS SGAIGNSGLA
481 NAGVLVSGVI NSGNTVSGLF NMSLVAITTP ALISGFFNTG SNMSGFFGGP PVFNLGLANR
541 GVVNILGNAN IGNYNILGSG NVGDFNILGS GNLGSQNILG SGNVGSFNIG SGNIGVFNVG
601 SGSLGNYNIG SGNLGIYNIG FGNVGDYNVG FGNAGDFNQG FANTGNNNIG FANTGNNNIG
661 IGLSGDNQQG FNIASGWNSG TGNSGLFNSG TNNVGIFNAG TGNVGIANSG TGNWGIGNPG
721 TDNTGILNAG SYNTGILNAG DFNTGFYNTG SYNTGGFNVG NTNTGNFNVG DTNTGSYNPG
781 DTNTGFFNPG NVNTGAFDTG DFNNGFLVAG DNQGQIAIDL SVTTPFIPIN EQMVIDVHNV
841 MTFGGNMITV TEASTVFPQT FYLSGLFFFG PVNLSASTLT VPTITLTIGG PTVTVPISIV
901 GALESRTITF LKIDPAPGIG NSTTNPSSGF FNSGTGGTSG FQNVGGGSSG VWNSGLSSAI
961 GNSGFQNLGS LQSGWANLGN SVSGFFNTST VNLSTPANVS GLNNIGTNLS GVFRGPTGTI
1021 FNAGLANLGQ LNIGSANLGD FNLGSGNVGS FNVFSGNQGS YNIGPANLGN YNIGFANLGN
1081 YNIGFGNAGD FNQGFANTGN NNIGFANTGN NNIGIGLSGD NQQGFNFAGG WNSGTANIGL
1141 FNSGTNNVGI GNSGTGNWGI GNSGSGNTGI GNTGSTNTGF FNTGIVNTGV ANAGSYNTGW
1201 YNTGDTNTGI ANLGDFNTGF YNTGNFSTGF ANQGDIATGA FITGDMGNGA FWRGDQQGLF
1261 SAGYRVHVPE IPAHVTVEVP VNIPITASFT NTVYSGITLE QINFGFTIDI AGIPLLAGAI
1321 SKAVLPPITG TGPAITVNIG DPGGSTAIRI PATASVGPFD VTFVNIAATT GFFNATTDPS
1381 SGFFNGGPGT VSGIANIGAN ISGFQNVANS ATSGFNNYGS LQSGLANLGD TVSGVFNTGI
1441 GAPANVSGMF NIGSNLAGFF HDQATGMSMF NLGLGNIGQF NVGFSNVGDS NAGLANIGSF
1501 NLGSGNLGSF NVFGGNQGSY NIGPANLGNY NIGLGNLGSY NFGFGNAGDF NLGFANTGNN
1561 NIGFANTGNN NIGIGLSGDN QQGFNFAGGW NSGSGNSGLF NSGTNNIGLF NSGTGNIGIG
1621 NSGTGNWGIA NTGDTNTGIF NTGDVNTGLL NAGNVNTGIF NTGHYNTGSF NAGSFNTAGF
1681 NPGSYNTGYL NTGSYNTGLA NSGDVNTGGF ITGNYSNGFW WRGDYQGLAG ISQTITVPDT
1741 AVPVKLHVPI FLDIPVTGTL GTFTVHGFRF PEITGDIFLI GIPFNAATLD AFSFPNISIV
1801 LPNIGINLGS GPDPLIDIAG TGGLLPIKIP LIDIPAAPGF GNSTTTPSSG FFNAGTGTVS
1861 GVGNVGSNSS GFFDLTSGSS GISGVQNFGE LISGGFNFGN TVSGLVNAST LGLSMPANLS
1921 GGGNVGATVA GFVNNTQILN LGFGNVGSGN VGHGNIGDSN VGLGNLGNAN VGHGNIGSFN
1981 VFSGNRGSYN IGPANLGNYN IGLGNLGSYN FGFGNAGDFN LGFANSGSNN IGFANTGNNN
2041 IGIGLSGHNQ QGFGSWNSGT ANTGLFNSGT NNIGLFNSGT GNIGIGNSGI GNTGIGNPGV
2101 GNTGLGNSGT GNWGLWNPGT GNMGVANVGT YNTGGYNVGS TNTGIANVGI ANTGSYNTGS
2161 TNTGSFNDGD FNTGFYNTGD YNTGFYNTGD VNTGAFIGGN FSNGAFWQSD HQGQWGAHYA
2221 ITVPQIPLLN FSLNIPVNIP IHLDFGTLAV NGFQIPAITL RALGVTHFSV GPIIVPRIAG
2281 TLPVIDINIG DPGGSSSIPI TITSGAGPVV IPLLDIPPAP GFGNSTTGPS SGFFNSGTGS
2341 SSGFGNVGAN NSGFWNTAFA GIGNSGLQNF GSLQSGWANL GNTVSGFYNT SAADFATPAN
2401 LSGLSNVGAD LTGVLRGPNG STFNAGLANL GQFNVGSANL GSANLGSANL GNSNVGFGNI
2461 GNANIGGANI GDFNVGIANT GPGLTAAVNN IGIGNTGNYN IGVGNTGNYN IGFGNTGNNN
2521 IGIGLSGDNQ IGFGPLNAGI ANMGLFNLGD NNFGMANAGN FNQGIANTGN NNIGLFNTGN
2581 NNVGIGLTGD GLSGFSSLNS GAGNTGFFNS GTANTGLFNS GTGNTGLFNS GTGNVGIGNM
2641 GTGGFGVGLS GDSQVGIGGT NSGSFNIGLF NSGTGNVGIG NSGTGNVGIG NTGTGNTGIG
2701 NSGNYNTGLL NAGLVNTGIA NPGNHNTGLF NIGTFNTGIA NPGHYNTGSY NTGSYNTGMA
2761 NAGDYGTGAF ITGSMNNGLL WRADRQGLLA ANYTITIERP AAFLNVDIPV NIPITGDITN
2821 VSIPAITFPR IDASGSVDIG ILSGTVLAPV GPITLHGGDA SAPLDTPIEI DFGPSPAINL
2881 NIGKPDGSTV INIVGGAGAG PISIPIIDLR PAPGFFNATT GPSSGFLNWG AGSASGLLNF
2941 GNNSGLYNFA TSSMGNSGFQ NYGSLQSGWA NLGNSISGIY NTGLGAPANV SGLLNIGTNL
3001 AGWLQNGPTE TTFSVGLANL GFWNLGSANI GNYNLGSANI GVYNLGSANI GDFNLGSANI
3061 GDFNLGSANI GSSNIGFGNV GPGLTAAIGN IGFGNTGNGN IGIGNTGTGN IGFGNTGNGN
3121 IGIGLTGDTM TGFGGWNSGT GNIGLFNSGT GNIGFGNSGT GNWGIGNSGD YNTGIGNTGS
3181 TNSGFFNTGL VNTGIGNSGD YNTGLFNAGN TNTGSFNPGD YNTGGFNPGN YNTGYFNPGN
3241 SNTGFANSGD VNTGAFNSGN YSNGFFWRGD YQGLGGFAYQ SAVSEIPWSY DIGSNIEIPI
3301 EGDINAITQD AFTIDEFEIP IKLRVSVCVI YIPFKGCVKH VSVTIPITTE HLGPYEIDAS
3361 TINPDQPIDT AFTQTLDFAG SGTVGAFPFG FGWQQSPGFF NSTTTPSSGF FNSGAGGASG
3421 FLNDAAAAVS GLGNVFTETS GFFNAGGVGN SGFQNFGNLL SGWANLGNTV SGFYNTSMLD
3481 LATQALISGF GNHGARLSGI LNNGSGP
//