LOCUS CAB5248051.1 882 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable iron-sulfur-binding reductase protein. ACCESSION LT708304-350 PROTEIN_ID CAB5248051.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0345C" /note="Mb0345c, -, len: 882 aa. Equivalent to Rv0338c, len: 882 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 882 aa overlap). Probable iron-sulphur-binding reductase (EC 1.-.-.-), possibly membrane-bound, equivalent to CAC32018.1|AL583925 probable iron-sulphur-binding reductase from Mycobacterium leprae (880 aa). Also highly similar to others e.g. T36608|5019323|CAB44376.1|AL078610 probable iron-sulfur-binding reductase from Streptomyces coelicolor (760 aa), FASTA scores: opt: 1658, E(): 0, (49.9% identity in 772 aa overlap); BAB07521.1|AP001520 iron-sulphur-binding reductase from Bacillus halodurans (700 aa). Contains PS00070 Aldehyde dehydrogenases cysteine active site and two of PS00198 4Fe-4S ferredoxins, iron-sulfur binding region signature. First of several possible start sites chosen. Protein product from Mb0345c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0345c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MTTQTLIRLI LGMSMTAVVG VFALRRVWWL YKLVMSGQPA SGRTDNLGTR IWTQISEVLG 61 QRRLLKWSIP GLAHFFTMWG FFILLTVYIE AYGLLFEERF HIPVIGRWDA LGFLQDFFAT 121 AVFLGITTFA IIRILRNPRE IGRSSRFYGS HNGGAWLVLL MIFNVIWTYV LVRGSAVNNG 181 TLPYGNGAFL SQLFGAILRP LGQPANEIIE TTALLLHIGV MLAFLILVLH SKHLHIFLAP 241 INVTFKRLPD GLGPLLPLEA DGKPIDFENP SEDAVFGRGK IEDFTWKGML DFATCTECGR 301 CQSQCPAWNT GKPLSPKLVI MDLRDHWMAK APYILGQKDA SAGGEAGHQE HHHVPESGFG 361 RVPGHGPEQA TRPLVGTEEQ GGVIDPDVLW SCVTCGACVE QCPVDIEHVD HIVDMRRYQV 421 MMESEFPSEL SVLFKNLETK GNPWGQNASD RTNWIDEVDF DVPVYGQDVD SFDGYEYLFW 481 VGCAGAYDDK AKKTTKAVAE LLAVAGVKYL VLGAGETCNG DSARRSGNEF LFQQLAQQAV 541 ETLDGLFEGV ETVDRKIVVT CPHCFNTIGK EYRQLGANYT VLHHTQLLNR LVRDKRLVPV 601 TPVSQDITYH DPCYLGRHNK VYEAPRELIG AAGASLTEMP RHADRSFCCG AGGARMWMEE 661 HIGKRINHER VDEALATDAT AIATACPFCR VMVTDGVNDR QEEAGRSGVE VLDVAQVLLG 721 SLDHDKAQLP AKGTAAKQAQ ERAPKAAPKA AAPVTPVEAP AEAPQAPAPA APAAPVKGLG 781 MAAGAKRPGA KKAAPTPAAP AAPAAPVKGL GIAAGAKRPG AKKTPPPAPG LAEPAAQPQP 841 EAKPQPEPAA PPKPQTDGDP AAPAAPVKGL GIARGARPPG KR //