LOCUS CAB5248040.1 449 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE CYTOCHROME P450 135A1 CYP135A1 protein. ACCESSION LT708304-339 PROTEIN_ID CAB5248040.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cyp135A1" /locus_tag="BQ2027_MB0334C" /note="Mb0334c, cyp135A1, len: 449 aa. Equivalent to Rv0327c, len: 449 aa, from Mycobacterium tuberculosis strain H37Rv, (99.8% identity in 449 aa overlap). Possible cyp135A1, cytochrome P450 (EC 1.14.-.-), similar to cytochrome P-450 monoxygenases and other cytochrome P-450 related enzymes e.g. FQ12609 PUTATIVE P450 MONOOXYGENASE (EC 1.14.14.1) (506 aa), FASTA scores: opt: 276, E() : 1.7e-11, (27.9% identity in 433 aa overlap). Also similar to other Mycobacterium tuberculosis proteins e.g. MTV039.06|Rv0568 PUTATIVE CYTOCHROME P450 (472 aa); MTCI5.10 cytochrome p450 FASTA score: (30.4% identity in 434 aa overlap). Contains cytochrome P450 cysteine heme-iron ligand signature (PS00086). BELONGS TO THE CYTOCHROME P450 FAMILY. Alternative start possible at 33706 but no RBS." BEGIN 1 MASTLTTGLP PGPRLPRYLQ SVLYLRFREW FLPAMHRKYG DVFSLRVPPY ADNLVVYTRP 61 EHIKEIFAAD PRSLHAGEGN HILGFVMGEH SVLMTDEAEY ARMRSLLMPA FTRAALRGYR 121 DMIASVAREH ITRWRPHATI NSLDHMNALT LDIILRVVFG VTDPKVKAEL TSRLQQIINI 181 HPAILAGVPY PSLKRMNPWK RFFHNQTKID EILYREIASR RIDSDLTART DVLSRLLQTK 241 DTPTKPLTDA ELRDQLITLL LAGHETTAAA LSWTLWELAH APEIQSQVVW AAVGGDDGFL 301 EAVLKEGMRR HTVIASTARK VTAPAEIGGW RLPAGTVVNT SILLAHASEV SHPKPTEFRP 361 SRFLDGSVAP NTWLPFGGGV RRCLGFGFAL TEGAVILQEI FRRFTITAAG PSKGETPLVR 421 NITTVPKHGA HLRLIPQRRL GGLGDSDPP //