LOCUS CAB5248038.1 226 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE TRANSCRIPTIONAL REGULATORY PROTEIN (POSSIBLY ARSR-FAMILY) protein. ACCESSION LT708304-337 PROTEIN_ID CAB5248038.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0332" /note="Mb0332, -, len: 226 aa. Equivalent to Rv0324, len: 226 aa, from Mycobacterium tuberculosis strain H37Rv, (99.6% identity in 226 aa overlap). Possible transcriptional regulator, arsR family, with its N-terminus similar to the N-terminus of other DNA-binding proteins e.g. P30346|MERR_STRLI probable mercury resistance operon from Streptomyces lividans (125 aa), FASTA scores: opt: 154, E(): 0.002, (32.2% identity in 90 aa overlap)), and its C-terminal part similar to hypothetical bacterial proteins e.g. P54510|YQHL_BACSU hypothetical 14.6 kd protein from Bacillus subtilis (126 aa), FASTA scores: opt: 159, E(): 0.00097, (35.5% identity in 76 aa overlap)). Most similar to AJ005575|SPE005575_2 ORF1 required for antibiotic production from Streptomyces peucetius (226 aa), FASTA scores: opt: 816, E(): 0, (60.7% identity in 211 aa overlap). Also similar in C-terminus to MTCY164.26 molybdopterin biosynthesis moeb protein from Mycobacterium tuberculosis FASTA score: (36.8% identity in 114 aa overlap). Protein product from Mb0332 detected using SWATH mass spectrometry." BEGIN 1 MAGQSDRKAA LLDQVARVGK ALANGRRLQI LDLLAQGERA VEAIATATGM NLTTASANLQ 61 ALKSGGLVEA RREGTRQYYR IAGEDVARLF ALVQVVADEH LADVAVAAAD VLGSPEDAIT 121 RAELLRRREA GEVTLVDVRP HEEYQAGHIP GAINIPIAEL ADRLAELAGD RDIVAYCRGA 181 YCVMAPDAVR IARDAGREVK RLDDGMLEWR LAGLPVDEGA PVGHGD //