LOCUS CAB5248033.1 222 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE PYRROLIDONE-CARBOXYLATE PEPTIDASE PCP (5-OXOPROLYL-PEPTIDASE) (PYROGLUTAMYL-PEPTIDASE I) (PGP-I) (PYRASE) protein. ACCESSION LT708304-332 PROTEIN_ID CAB5248033.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="pcp" /locus_tag="BQ2027_MB0327" /note="Mb0327, pcp, len: 222 aa. Equivalent to Rv0319, len: 222 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 222 aa overlap). Probable pcp, pyrrolidone-carboxylate peptidase (EC 3.4.19.3), highly similar to others e.g. PCP_PSEFL|P42673 pyrrolidone-carboxylate peptidase from Pseudomonas fluorescens (213 aa), FASTA scores: opt: 478, E(): 7.5e-25, (40.2% identity in 219 aa overlap). BELONGS TO PEPTIDASE FAMILY C15 (THIOL PROTEASE). Protein product from Mb0327 detected using SWATH mass spectrometry. Mb0327 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MSKVLVTGFG PYGVTPVNPA QLTAEELDGR TIAGATVISR IVPNTFFESI AAAQQAIAEI 61 EPALVIMLGE YPGRSMITVE RLAQNVNDCG RYGLADCAGR VLVGEPTDPA GPVAYHATVP 121 VRAMVLAMRK AGVPADVSDA AGTFVCNHLM YGVLHHLAQK GLPVRAGWIH LPCLPSVAAL 181 DHNLGVPSMS VQTAVAGVTA GIEAAIRQSA DIREPIPSRL QI //