LOCUS CAB5248031.1 256 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 possible glycerophosphoryl diester phosphodiesterase glpq2 (glycerophosphodie ster phosphodiesterase) protein. ACCESSION LT708304-330 PROTEIN_ID CAB5248031.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="glpQ2" /locus_tag="BQ2027_MB0325C" /note="Mb0325c, glpQ2, len: 256 aa. Equivalent to Rv0317c, len: 256 aa (start uncertain, chosen by homology), from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 256 aa overlap). Possible glpQ2, glycerophosphoryl diester phosphodiesterase (EC 3.1.4.46), similar to others e.g. E75317|6459876|AAF11631.1|AE002044_4 glycerophosphoryl diester phosphodiesterase from Deinococcus radiodurans (285 aa); P10908|UGPQ_ECOLI from Escherichia coli (247 aa), FASTA scores: opt: 220, E(): 5.2e-07, (28.0% identity in 250 aa overlap). Also similar to MTCY01A6.27 from Mycobacterium tuberculosis FASTA score: (27.5% identity in 247 aa overlap). Protein product from Mb0325c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0325c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MEFLRHGGRI AMAHRGFTSF RLPMNSMGAF QEAAKLGFRY IETDVRATRD GVAVILHDRR 61 LAPGVGLSGA VDRLDWRDVR KAQLGAGQSI PTLEDLLTAL PDMRVNIDIK AASAIEPTVN 121 VIERCNAHNR VLIGSFSERR RRRALRLLTK RVASSAGTGA LLAWLTARPL GSRAYAWRMM 181 RDIDCVQLPS RLGGVPVITP ARVRGFHAAG RQVHAWTVDE PDVMHTLLDM DVDGIITDRA 241 DLLRDVLIAR GEWDGA //