LOCUS CAB5248030.1 204 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE MUCONOLACTONE ISOMERASE protein. ACCESSION LT708304-329 PROTEIN_ID CAB5248030.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0324" /note="Mb0324, -, len: 204 aa. Equivalent to Rv0316, len: 204 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 204 aa overlap). Possible muconolactone isomerase (EC 5.3.3.-), showing weak similarity with some muconolactone isomerases e.g. O33947|CTC1_ACILW MUCONOLACTONE DELTA-ISOMERASE 1 (MIASE 1)(96 aa), FASTA scores: opt: 179, E(): 3.9e-05, (32.6% identity in 92 aa overlap). Protein product from Mb0324 detected using SWATH mass spectrometry. Mb0324 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MEFLVTMTTR VPDSMPADAV ERVRAREAAR SRELAAQGKL LRLWRPPLRP GEWRTLGLFA 61 ADDNGELEQL LASMPPRSWR TDDVTPLGAH PNDPVGQGIT IAPGKGPEFL IATTIMVPPG 121 TPAQVVDDTV AREARRAPEL AGRGHLVRLW ALPDGPDGQR TLGLWRARDP GELMAILESL 181 PLAGWMTIET TPLSPHPDDP IRMP //