LOCUS CAB5248029.1 294 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE BETA-1,3-GLUCANASE PRECURSOR protein. ACCESSION LT708304-328 PROTEIN_ID CAB5248029.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0323" /note="Mb0323, -, len: 294 aa. Equivalent to Rv0315, len: 294 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 294 aa overlap). Possible beta-1,3-glucanase precursor (EC 3.2.1.-) (has hydrophobic stretch in its N-terminal part), similar to others e.g. Q51333|AAC44371.1 BETA-1,3-GLUCANASE II A from Oerskovia xanthineolytica (306 aa), FASTA scores: opt: 76, E(): 3e-14, (34.1% identity in 302 aa overlap); and AAC38290.1|AF052745 beta-1,3-glucanase II from Oerskovia xanthineolytica (435 aa). Contains glycosyl hydrolases family 16 active site signature (PS01034). Protein product from Mb0323 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0323 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MLMPEMDRRR MMMMAGFGAL AAALPAPTAW ADPSRPAAPA GPTPAPAAPA AATGGLLFHD 61 EFDGPAGSVP DPSKWQVSNH RTPIKNPVGF DRPQFFGQYR DSRQNVFLDG NSNLVLRATR 121 EGNRYFGGLV HGLWRGGIGT TWEARIKFNC LAPGMWPAWW LSNDDPGRSG EIDLIEWYGN 181 GTWPSGTTVH ANPDGTAFET CPIGVDGGWH NWRVTWNPSG MYFWLDYADG IEPYFSVPAT 241 GIEDLNEPIR EWPFNDPGYT VFPVLNLAVG GSGGGDPATG SYPQEMLVDW VRVF //