LOCUS CAB5248022.1 238 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CONSERVED INTEGRAL MEMBRANE PROTEIN protein. ACCESSION LT708304-321 PROTEIN_ID CAB5248022.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0316" /note="Mb0316, -, len: 238 aa. Equivalent to Rv0308, len: 238 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 238 aa overlap). Probable conserved integral membrane protein, with C-terminus highly similar to C-terminus of other integral membrane proteins or phosphatases e.g. AAK25788.1|AF336822_1|13430250|AAK25789. 1|AF336823_1 putative phosphatase from Streptococcus pyogenes (201 aa); Q06074 HYPOTHETICAL 24.9 KD PROTEIN (216 aa), FASTA scores: opt: 209, E(): 2e-07, (27.9% identity in 140 aa overlap). Could be a phosphatase. Protein product from Mb0316 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0316 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MTRPQALLAV SLAFVATAVY AVMWVGHSQD WGWLHSFDWS LLNAAHDIGI KNPAWVRFWD 61 GVSLILGPVV LRPLGLLAAM VALAKRKIRI ALLLLACLPL NAIMTIAAKS VAHRPRPATA 121 LVSAHSTSFP SGHALEATAS VLALLTVLLP MLHSRFTRHI AITVGALCVL TVGVARVALN 181 VHHPTDVVAG WALGYLYFLV CLCVFRPPSI FGAQRASHAL SPPVEVSRQP EPEVDTAR //