LOCUS CAB5248010.1 465 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE SULFATASE protein. ACCESSION LT708304-309 PROTEIN_ID CAB5248010.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0304C" /note="Mb0304c, -, len: 465 aa. Equivalent to Rv0296c, len: 465 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 465 aa overlap). Probable sulfatase, possibly an aryl-/steryl-sulfatase (EC 3.1.6.-) or a sulfamidase (sulfohydrolase) (sulphamidase) (EC 3.10.1.-). Similar to various hydrolases e.g. AAG41945.1|AF304053_1|AF304053 heparan N-sulfatase from Mus musculus (502 aa); NP_061292.1|6851181|AAF29460.1|AF153827_1|AF153827 N-sulfoglucosamine sulfohydrolase (sulfamidase) (sulphamidase) from Mus musculus (502 aa); AAG17206.1|AF217203_1|AF217203 heparan sulfate sulfamidase from Canis familiaris (507 aa); P08842|STS_HUMAN|1360652 STERYL-SULFATASE PRECURSOR (EC 3.1.6.2) (STEROID SULFATASE) (STERYL-SULFATE SULFOHYDROLASE) (ARYLSULFATASE C) (ASC) from Homo sapiens (583 aa); ARSB_FELCA|P33727 arylsulfatase B precursor (EC 3.1.6.1) (535 aa), FASTA scores: opt: 231, E(): 1.7e-08, (30.3% identity in 261 aa overlap). Also similarity with 4 others sulfatases in Mycobacterium tuberculosis. Contains sulfatases signature 1 (PS00523). Note that previously known as atsG. Protein product from Mb0304c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0304c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." BEGIN 1 MTSERATGQR ENLLIVHWHD LGRYLGVYHH PDVYSPRLDR LAAEGILFTR AHATAPLCTP 61 SRGSLFTGRY PQSNGLVGLA HHGWEYRTGV QTLPQLLSES GWYSALFGMQ HETSYPKRLG 121 FDEFDVSNSY CEYVVAKAQD WLHNRVPALD GQRFLLTAGF FETHRPYPHE RYRPADSAAV 181 ELPDYLPDTP EVRQDVAEFY GSIATADEAV GRLLDTLADT GLDASTWVVF VTDHGPAFPR 241 AKSTLYDAGT GIALIIRPPT RRAMAPRVYD ELFSGVDLVP TLLDLLRLEV PADVEGVSHA 301 PALLAPDTEN AAVRDHVYTA KTYHDSFDPI RAIRTKEYSY IENYAPRPLL DLPWDIQESP 361 AGMAVAPLVK APRPQRELYD LRADPTETNN LLAGDDSTQG VAAIAADLAV RLHDWRQRTA 421 DVIPSDFAGS RIAERYTETY LRIHRKTPTG RSAIAADRGI DEHCS //