LOCUS CAB5248008.1 261 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE TRANS-ACONITATE METHYLTRANSFERASE TAM protein. ACCESSION LT708304-307 PROTEIN_ID CAB5248008.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="tam" /locus_tag="BQ2027_MB0302" /note="Mb0302, tam, len: 261 aa. Equivalent to Rv0294, len: 261 aa, from Mycobacterium tuberculosis strain H37Rv (100.0% identity in 261 aa overlap). Probable tam, trans-aconitate methyltransferase (EC 2.1.1.-), similar to others e.g. P76145|TAM_ECOLI|7465793|B64906|B1519 TRANS-ACONITATE METHYLTRANSFERASE from Escherichia coli strain K12 (252 aa), FASTA scores: opt: 649, E(): 0, (39.3 identity in 252 aa overlap). BELONGS TO THE METHYLTRANSFERASE SUPERFAMILY. Protein product from Mb0302 detected using SWATH mass spectrometry. Mb0302 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MWDPDVYLAF SGHRNRPFYE LVSRVGLERA RRVVDLGCGP GHLTRYLARR WPGAVIEALD 61 SSPEMVAAAA ERGIDATTGD LRDWKPKPDT DVVVSNAALH WVPEHSDLLV RWVDELAPGS 121 WIAVQIPGNF ETPSHAAVRA LARREPYAKL MRDIPFRVGA VVQSPAYYAE LLMDTGCKVD 181 VWETTYLHQL TGEHPVLDWI TGSALVPVRE RLSDESWQQF RQELIPLLND AYPPRADGST 241 IFPFRRLFMV AEVGGARRSG G //