LOCUS CAB5248000.1 513 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 ppe family protein ppe4 protein. ACCESSION LT708304-299 PROTEIN_ID CAB5248000.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="PPE4" /locus_tag="BQ2027_MB0294" /note="Mb0294, PPE4, len: 513 aa. Equivalent to Rv0286, len: 513 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 513 aa overlap). Member of the Mycobacterium tuberculosis PPE family, similar to others e.g. AL0212|MTV012_32 from Mycobacterium tuberculosis (434 aa), FASTA scores: opt: 958, E(): 0, (43.5% identity in 522 aa overlap). Protein product from Mb0294 detected using SWATH mass spectrometry. Mb0294 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MAAPIWMASP PEVHSALLSN GPGPGSLVAA ATAWSQLSAE YASTAAELSG LLGAVPGWAW 61 QGPSAEWYVA AHLPYVAWLT QASADAAGAA AQHEAAAAAY TTALAAMPTL AELAANHVIH 121 TVLVATNFFG INTIPITLNE ADYVRMWLQA AAVMGLYQAA SGAALASAPR TVPAPTVMNP 181 GGGAASTVGA VNPWQWLLAL LQQLWNAYTG FYGWMLQLIW QFLQDPIGNS IKIIIAFLTN 241 PIQALITYGP LLFALGYQIF FNLVGWPTWG MILSSPFLLP AGLGLGLAAI AFLPIVLAPA 301 VIPPASTPLA AAAVAAGSVW PAVSMAVTGA GTAGAATPAA GAAPSAGAAP APAAPATASF 361 AYAVGGSGDW GPSLGPTVGG RGGIKAPAAT VPAAAAAAAT RGQSRARRRR RSELRDYGDE 421 FLDMDSDSGF GPSTGDHGAQ ASERGAGTLG FAGTATKERR VRAVGLTALA GDEFGNGPRM 481 PMVPGTWEQG SNEPEAPDGS GRGGGDGLPH DSK //