LOCUS CAB5247982.1 560 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE FATTY-ACID-COA LIGASE FADD2 (FATTY-ACID-COA SYNTHETASE) (FATTY-ACID-COA SYNTHASE) protein. ACCESSION LT708304-281 PROTEIN_ID CAB5247982.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadD2" /locus_tag="BQ2027_MB0276" /note="Mb0276, fadD2, len: 560 aa. Equivalent to Rv0270, len: 560 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 560 aa overlap). Probable fadD2, fatty-acid-CoA synthetase (EC 6.2.1.-), similar to many e.g. LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase from Escherichia coli (561 aa), FASTA scores: opt: 544, E(): 2.9e-26, (27.7% identity in 535 aa overlap). Also similar to others from Mycobacterium tuberculosis e.g. MTCY493_2, MTCY8D5_9, MTCY6G11_8, etc. Contains PS00455 Putative AMP-binding domain signature. Protein product from Mb0276 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0276 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MPNLTDLPGQ AVSKLQKSIG QYVARGTAEL HYLRKIIESG AIGLEPPLNY AALAADIRKW 61 GEVGMLPSHN ARRAPNRAAV IDEEGTLTFS ELDEAAHAVA NGLLAKGVRA GDGVAILARN 121 HRWFVIANYG AARVGARIIL LNSEFSGPQI KEVSDREGAK VIIYDDEYTK AVSLAQPPLG 181 KLRALGVNPD DDKPSGSSDE TLAELIAHSS TAPAPKASRR ASIIILTSGT TGTPKGANRN 241 TPPTLAPIGG ILSHVPFKAG EVTLLPSPMF HALGYMHAAL AMFLGSTLVL RRRFKPALVL 301 EDIEKHKATS MVVVPVMLSR ILDQLEKTEP KPDLSSLKIV FVSGSQLGAE LATRALGDLG 361 PVIYNMYGST EVAFATIAGP KDLQFNPSTV GPVVKGVTVK ILDENGNEVP QGAVGRIFVG 421 NAFPFEGYTG GGGKQIIDGL LSSGDVGYFD ERGLLYVSGR DDEMIVSGGE NVFPAEVEDL 481 ISGHPDVVEA AAIGVDDKEF GARLRAFVVK KPGADLDEDT IKQYVRDHLA RYKVPREVIF 541 LDELPRNPTG KVLKRELRKL //