LOCUS       CAB5247982.1             560 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            FATTY-ACID-COA LIGASE FADD2 (FATTY-ACID-COA SYNTHETASE)
            (FATTY-ACID-COA SYNTHASE) protein.
ACCESSION   LT708304-281
PROTEIN_ID  CAB5247982.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="fadD2"
                     /locus_tag="BQ2027_MB0276"
                     /note="Mb0276, fadD2, len: 560 aa. Equivalent to Rv0270,
                     len: 560 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 560 aa overlap). Probable fadD2,
                     fatty-acid-CoA synthetase (EC 6.2.1.-), similar to many
                     e.g. LCFA_ECOLI|P29212 long-chain-fatty-acid--CoA ligase
                     from Escherichia coli (561 aa), FASTA scores: opt: 544,
                     E(): 2.9e-26, (27.7% identity in 535 aa overlap). Also
                     similar to others from Mycobacterium tuberculosis e.g.
                     MTCY493_2, MTCY8D5_9, MTCY6G11_8, etc. Contains PS00455
                     Putative AMP-binding domain signature. Protein product
                     from Mb0276 detected using shotgun mass spectrometry and
                     SWATH mass spectrometry. Mb0276 found to be expressed
                     during exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MPNLTDLPGQ AVSKLQKSIG QYVARGTAEL HYLRKIIESG AIGLEPPLNY AALAADIRKW
       61 GEVGMLPSHN ARRAPNRAAV IDEEGTLTFS ELDEAAHAVA NGLLAKGVRA GDGVAILARN
      121 HRWFVIANYG AARVGARIIL LNSEFSGPQI KEVSDREGAK VIIYDDEYTK AVSLAQPPLG
      181 KLRALGVNPD DDKPSGSSDE TLAELIAHSS TAPAPKASRR ASIIILTSGT TGTPKGANRN
      241 TPPTLAPIGG ILSHVPFKAG EVTLLPSPMF HALGYMHAAL AMFLGSTLVL RRRFKPALVL
      301 EDIEKHKATS MVVVPVMLSR ILDQLEKTEP KPDLSSLKIV FVSGSQLGAE LATRALGDLG
      361 PVIYNMYGST EVAFATIAGP KDLQFNPSTV GPVVKGVTVK ILDENGNEVP QGAVGRIFVG
      421 NAFPFEGYTG GGGKQIIDGL LSSGDVGYFD ERGLLYVSGR DDEMIVSGGE NVFPAEVEDL
      481 ISGHPDVVEA AAIGVDDKEF GARLRAFVVK KPGADLDEDT IKQYVRDHLA RYKVPREVIF
      541 LDELPRNPTG KVLKRELRKL
//