LOCUS CAB5247981.1 397 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 ATP- dependent DNA ligase (EC protein. ACCESSION LT708304-280 PROTEIN_ID CAB5247981.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0275C" /EC_number="6.5.1.1" /note="Mb0275c, -, len: 397 aa. Equivalent to Rv0269c, len: 397 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 397 aa overlap). Conserved hypothetical protein, highly similar to AL079355|SC4C6_19 hypothetical protein from Streptomyces coelicolor (341 aa), FASTA scores: opt: 1019, E(): 0, (46.5% identity in 344 aa overlap), and similar to other proteins e.g. CAC49016.1|AL603644 putative ATP-dependent DNA ligase protein from Sinorhizobium meliloti (636 aa); O34398 YKOU PROTEIN from Bacillus subtilis (611 aa), FASTA score: (27.2% identity in 283 aa overlap). Also similar to proteins from Mycobacterium tuberculosis e.g. Rv3062, Rv3731 (both DNA ligases), and Rv0938, Rv3730c. Protein product from Mb0275c detected using SWATH mass spectrometry. Mb0275c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MSRMAAPVSL DVHGRQVIVT HPGRVVFPAH NDRKGYTKFD LVRYYLAVAE GAMRGVAGRP 61 MILKRFVKGI SAEAVFQKRA PANRPDWVDV AELHYASGRS AAEAVIHDAA GLAWVINLGC 121 VDLNPHPVLA GDLDHPDELR VDLDPMPGVA WQRVVEVALV VREVLEDYGL TAWPKTSGSR 181 GFHVYARIAP CWSFPQVRLA AQTVAREVER RLPDAATSRW WKEEREGVFV DFNQNAKDRT 241 VASAYSVRAT PDARVSTPLH WEEVPGCDPA VFTMATVPSR LADIGDPWAG MDDAVGRLDR 301 LLMLAEELGP PQKAQSAKPL IEIARAKTRA EAMAALDIWR DRYPGAAALL RPADVLVDGM 361 RGPSSIWYRI RINLQHVPAD QRPPQEELIA DYSPWPR //