LOCUS CAB5247975.1 300 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 Allophanate hydrolase 2 subunit 2 (EC protein. ACCESSION LT708304-274 PROTEIN_ID CAB5247975.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0269C" /EC_number="3.5.1.54" /note="Mb0269c, -, len: 300 aa. Equivalent to Rv0263c, len: 300 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 300 aa overlap). Conserved hypothetical protein, equivalent to NP_302634.1|NC_002677 conserved hypothetical protein from Mycobacterium leprae (305 aa). Also similar to others e.g. AL121596|SC51A_21 hypothetical protein from Streptomyces coelicolor (285 aa), FASTA scores: opt: 714, E(): 0, (45.3% identity in 289 aa overlap); NP_233164.1|NC_002506 conserved hypothetical protein from Vibrio cholerae (309 aa); NP_406216.1|NC_003143 conserved hypothetical protein from Yersinia pestis (316 aa); YH30_HAEIN|P44298|hi1730 hypothetical protein from Haemophilus influenzae (309 aa), FASTA scores: opt: 430, E(): 3e-20, (29.6% identity in 284 aa overlap); etc. Also similar to carboxylases eg NP_415240.1|NC_000913|P75745|YBGK_ECOLI putative carboxylase from Escherichia coli strain K12 (310 aa), FASTA score: (34.6% identity in 286 aa overlap); NP_459698.1|NC_003197 putative carboxylase from Salmonella typhimurium (310 aa); and to middle part of NP_420636.1|NC_002696 urea amidolyase-related protein from Caulobacter crescentus (1207 aa). Protein product from Mb0269c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0269c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MTTLEILRSG PLALVEDLGR AGLAHLGVGR SGAADRRSHT LANRLVANPD DWATVEVTFG 61 GFSARVRGGD VDIAVTGADT DPTVNGIMVG TNSIHHVRDG QVISLGTPRA GLRTYLAVRG 121 GVCVEPVLGS RSYDVMSAIG PSPLRAGDVL PVGEHTDDYP ELDQAPVAAI EEHLVELRVV 181 PGPRDDWLVD PDALVHTIWM ASNRSDRVGM RLQGRPLQHR WPDRQLPGEG VTRGAIQVPP 241 NGLPVILGPD HPITGSYPVV GVITDEDIDK VAQIRPGQYV RLHWARPRSR LPGQGVTQAW //