LOCUS CAB5247966.1 174 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable bifunctional cobalamin biosynthesis protein cobu: cobinamide kinase + cobinamide phosphate guanylyltransferase protein. ACCESSION LT708304-265 PROTEIN_ID CAB5247966.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="cobU" /locus_tag="BQ2027_MB0260C" /note="Mb0260c, cobU, len: 174 aa. Equivalent to Rv0254c, len: 174 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 174 aa overlap). Probable cobU, cobalamin biosynthesis protein including a cobinamide kinase (EC 2.-.-.-) and cobinamide phosphate guanylyltransferase (EC 2.-.-.-). Highly similar to many e.g. Q05599|COBU_SALTY COBINAMIDE KINASE / COBINAMIDE PHOSPHATE GUANYLYLTRANSFERASE from Salmonella typhimurium (181 aa), FASTA scores: opt: 308, E(): 1.1e-14, (38.7% identity in 181 aa overlap); P46886|COBU_ECOLI|B1993|Z3153|ECS2788 Bifunctional cobalamin biosynthesis protein cobU from Escherichia coli strains K12 and O157:H7 (181 aa); part of AL096872|SC5F7_10 from Streptomyces coelicolor (397 aa), FASTA scores: opt: 445, E(): 3.6e-23, (46.0% identity in 176 aa overlap); etc. Contains PS00017 ATP/GTP-binding site motif A (P-loop). Protein product from Mb0260c detected using SWATH mass spectrometry. Mb0260c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MRILVTGGVR SGKSTHAEAL LGDAADVVYV APGRPAAGSD PDWDARVALH RARRPPTWLT 61 VETADVATAL SEARSPVLVD CLGTWLTAIM DGEALWSAAT ADVYAVLEAR LDGLCAALTG 121 LPTAIVVTNE VGLGVVPSHS SGVLFRDLLG TINRRVAAVC DEVHLVIAGR VLKL //