LOCUS CAB5247961.1 273 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable succinate dehydrogenase [membrane anchor subunit] (succinic dehydrogenase) protein. ACCESSION LT708304-260 PROTEIN_ID CAB5247961.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0255C" /note="Mb0255c, -, len: 273 aa. Equivalent to Rv0249c, len: 273 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 273 aa overlap). Probable succinate dehydrogenase, membrane-anchor subunit for succinate dehydrogenase encoded by Rv0247c and Rv0248c. Highly similar to AC44315.1|AL596043 putative integral membrane protein from Streptomyces coelicolor (278 aa). NOTE THAT SUCCINATE DEHYDROGENASE FORMS GENERALLY PART OF AN ENZYME COMPLEX CONTAINING FOUR SUBUNITS: A FLAVOPROTEIN (Rv0248c ?), AN IRON-SULFUR (Rv0247c ?), AND TWO HYDROPHOBIC ANCHOR PROTEINS (Rv0249c ?). Protein product from Mb0255c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0255c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MSAPTANRPA IGVFTPTRAQ IPERTLRTDL WWLPPLLTNL GLLAFICYAT TRAFWGSQYW 61 VEKYHYLTPF YSPCVSASCQ PGASHLGVWF GHFPGWIPLG AMVLPFLLGF RLTCYYYRKA 121 YYRSVWQSPT SCAVPEPRAH YTGETRLPLI VQNTHRYFFY IAVVVSLINT YDAIAAFHSP 181 SGFGFGLGNV ILTINVVLLW AYTISCHSCR HATGGRLKHF SKHPVRYWIW TQVSKLNTRH 241 MQFAWITLGT LALTDFYIML VASGSITDLR FIG //