LOCUS       CAB5247960.1             646 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            SUCCINATE DEHYDROGENASE [IRON-SULFUR SUBUNIT] (SUCCINIC
            DEHYDROGENASE) protein.
ACCESSION   LT708304-259
PROTEIN_ID  CAB5247960.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /locus_tag="BQ2027_MB0254C"
                     /note="Mb0254c, -, len: 646 aa. Equivalent to Rv0248c,
                     len: 646 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 646 aa overlap). Probable succinate
                     dehydrogenase, flavoprotein subunit (EC 1.3.99.1), highly
                     similar to flavoprotein subunit of various succinate
                     dehydrogenases e.g. M88696|RIRSDHA_1 flavoprotein from
                     Rickettsia prowazekii (596 aa), FASTA scores: opt: 651,
                     E(): 0, (34.6 % identity in 598 aa overlap). Also similar
                     to truncated U00022_17 flavoprotein from Mycobacterium
                     leprae (401 aa), FASTA scores: opt: 677, E(): 0, (39.0%
                     identity in 423 aa overlap). NOTE THAT SUCCINATE
                     DEHYDROGENASE FORMS GENERALLY PART OF AN ENZYME COMPLEX
                     CONTAINING FOUR SUBUNITS: A FLAVOPROTEIN (Rv0248c ?), AN
                     IRON-SULFUR (Rv0247c ?), AND TWO HYDROPHOBIC ANCHOR
                     PROTEINS (Rv0249c ?). Protein product from Mb0254c
                     detected using shotgun mass spectrometry and SWATH mass
                     spectrometry. Mb0254c found to be expressed during
                     exponential growth in Sauton's minimal media by
                     RNA-sequencing."
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MVEVERHSYD VVVIGAGGAG LRAVIEARER GLKVAVVCKS LFGKAHTVMA EGGCAAAMGN
       61 ANPKDNWKTH FGDTMRGGKF LNNWRMAELH AKEAPDRVWE LETYGALFDR TDDGRISQRN
      121 FGGHTYPRLA HVGDRTGLEL IRTLQQKVVS LQQEDHAELG DYEARIKVFA ECTITELLKD
      181 QGAIAGAFGY WRESGRFIVF EAPAVVLATG GIGKSFKVTS NSWEYTGDGH ALALRAGATL
      241 INMEFVQFHP TGMVWPPSVK GILVTEGVRG DGGVLKNSEN SRFMFDYIPP VFKGQYAETE
      301 EEADQWLKDN DSARRTPDLL PRDEVARAIN SEVKAGRGTP HGGVYLDIAS RLTPAEIKRR
      361 LPSMYHQFKE LAEVDITTQA MEVGPTCHYV MGGVEVDADT GAATVPGLFA AGECAGGMHG
      421 SNRLGGNSLS DLLVFGRRAG LGAADYVRAL SSRPAVSAEA IDAAAQQALS PFEGPKDGSA
      481 PENPYALHMD LQYVMNDLVG IIRNADEISR ALTLLAELWS RYHNVLVEGH RQYNPGWNLS
      541 IDLRNMLLVS ECVARAALQR TESRGGHTRD DHPGMDPNWR RILLVCRATE TMGTGGSGSG
      601 DSNCHINVTQ QLQTPMRPDL LELFEISELE KYYTDEELAE HPGRRG
//