LOCUS CAB5247960.1 646 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE SUCCINATE DEHYDROGENASE [IRON-SULFUR SUBUNIT] (SUCCINIC DEHYDROGENASE) protein. ACCESSION LT708304-259 PROTEIN_ID CAB5247960.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0254C" /note="Mb0254c, -, len: 646 aa. Equivalent to Rv0248c, len: 646 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 646 aa overlap). Probable succinate dehydrogenase, flavoprotein subunit (EC 1.3.99.1), highly similar to flavoprotein subunit of various succinate dehydrogenases e.g. M88696|RIRSDHA_1 flavoprotein from Rickettsia prowazekii (596 aa), FASTA scores: opt: 651, E(): 0, (34.6 % identity in 598 aa overlap). Also similar to truncated U00022_17 flavoprotein from Mycobacterium leprae (401 aa), FASTA scores: opt: 677, E(): 0, (39.0% identity in 423 aa overlap). NOTE THAT SUCCINATE DEHYDROGENASE FORMS GENERALLY PART OF AN ENZYME COMPLEX CONTAINING FOUR SUBUNITS: A FLAVOPROTEIN (Rv0248c ?), AN IRON-SULFUR (Rv0247c ?), AND TWO HYDROPHOBIC ANCHOR PROTEINS (Rv0249c ?). Protein product from Mb0254c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0254c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MVEVERHSYD VVVIGAGGAG LRAVIEARER GLKVAVVCKS LFGKAHTVMA EGGCAAAMGN 61 ANPKDNWKTH FGDTMRGGKF LNNWRMAELH AKEAPDRVWE LETYGALFDR TDDGRISQRN 121 FGGHTYPRLA HVGDRTGLEL IRTLQQKVVS LQQEDHAELG DYEARIKVFA ECTITELLKD 181 QGAIAGAFGY WRESGRFIVF EAPAVVLATG GIGKSFKVTS NSWEYTGDGH ALALRAGATL 241 INMEFVQFHP TGMVWPPSVK GILVTEGVRG DGGVLKNSEN SRFMFDYIPP VFKGQYAETE 301 EEADQWLKDN DSARRTPDLL PRDEVARAIN SEVKAGRGTP HGGVYLDIAS RLTPAEIKRR 361 LPSMYHQFKE LAEVDITTQA MEVGPTCHYV MGGVEVDADT GAATVPGLFA AGECAGGMHG 421 SNRLGGNSLS DLLVFGRRAG LGAADYVRAL SSRPAVSAEA IDAAAQQALS PFEGPKDGSA 481 PENPYALHMD LQYVMNDLVG IIRNADEISR ALTLLAELWS RYHNVLVEGH RQYNPGWNLS 541 IDLRNMLLVS ECVARAALQR TESRGGHTRD DHPGMDPNWR RILLVCRATE TMGTGGSGSG 601 DSNCHINVTQ QLQTPMRPDL LELFEISELE KYYTDEELAE HPGRRG //