LOCUS       CAB5247959.1             248 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            SUCCINATE DEHYDROGENASE [IRON-SULFUR SUBUNIT] (SUCCINIC
            DEHYDROGENASE) protein.
ACCESSION   LT708304-258
PROTEIN_ID  CAB5247959.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /locus_tag="BQ2027_MB0253C"
                     /note="Mb0253c, -, len: 248 aa. Equivalent to Rv0247c,
                     len: 248 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 248 aa overlap). Probable succinate
                     dehydrogenase, iron-sulfur subunit (EC 1.3.99.1), highly
                     similar to CAC44313.1|AL596043 putative succinate
                     dehydrogenase iron-sulfur subunit from Streptomyces
                     coelicolor (259 aa); and similar to iron-sulphur protein
                     subunits of fumarate reductase or succinate dehydrogenases
                     from many bacteria e.g. NP_147618.1|7521083|B72691
                     fumarate reductase iron-sulfur protein from Aeropyrum
                     pernix (305 aa); NP_069516.1|2649932|AAB90556.1|AE001057
                     succinate dehydrogenase iron-sulfur subunit B (sdhB) from
                     Archaeoglobus fulgidus (236 aa); etc. Also similar to
                     Q10761|FRDB_MYCTU|7431693|F70762 FUMARATE REDUCTASE
                     IRON-SULFUR PROTEIN from Mycobacterium tuberculosis (247
                     aa), FASTA scores: opt: 358, E():1e-16, (31.3% identity in
                     214 aa overlap). Contains PS00197 2Fe-2S ferredoxins,
                     iron-sulfur binding region signature. NOTE THAT SUCCINATE
                     DEHYDROGENASE FORMS GENERALLY PART OF AN ENZYME COMPLEX
                     CONTAINING FOUR SUBUNITS: A FLAVOPROTEIN (Rv0248c ?), AN
                     IRON-SULFUR (Rv0247c ?), AND TWO HYDROPHOBIC ANCHOR
                     PROTEINS (Rv0249c ?). Protein product from Mb0253c
                     detected using shotgun mass spectrometry. Mb0253c found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing (TPM > 20)."
                     /experiment="experimental evidence, no additional details
                     recorded"
BEGIN
        1 MTYSASMRVW RGDESCGELR EFTVEVNEGE VVLDVILRLQ QTQTPDLAVR WNCKAGKCGS
       61 CSAEINGKPR LMCMTRMSTF DEDEIVTVTP MRTFPVIRDL VTDVSFNYQK AREIPSFAPP
      121 KELQPSEYRM AQVDVARSQE FRKCIECFLC QNVCHVVRDH EENKDAFAGP RFLMRIAELE
      181 MHPLDTRDRR SQAQEEHGLG YCNITKCCTE VCPENIKITD NALIPMKERV ADRKYDPVVW
      241 LGSKLFRR
//