LOCUS CAB5247959.1 248 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE SUCCINATE DEHYDROGENASE [IRON-SULFUR SUBUNIT] (SUCCINIC DEHYDROGENASE) protein. ACCESSION LT708304-258 PROTEIN_ID CAB5247959.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0253C" /note="Mb0253c, -, len: 248 aa. Equivalent to Rv0247c, len: 248 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 248 aa overlap). Probable succinate dehydrogenase, iron-sulfur subunit (EC 1.3.99.1), highly similar to CAC44313.1|AL596043 putative succinate dehydrogenase iron-sulfur subunit from Streptomyces coelicolor (259 aa); and similar to iron-sulphur protein subunits of fumarate reductase or succinate dehydrogenases from many bacteria e.g. NP_147618.1|7521083|B72691 fumarate reductase iron-sulfur protein from Aeropyrum pernix (305 aa); NP_069516.1|2649932|AAB90556.1|AE001057 succinate dehydrogenase iron-sulfur subunit B (sdhB) from Archaeoglobus fulgidus (236 aa); etc. Also similar to Q10761|FRDB_MYCTU|7431693|F70762 FUMARATE REDUCTASE IRON-SULFUR PROTEIN from Mycobacterium tuberculosis (247 aa), FASTA scores: opt: 358, E():1e-16, (31.3% identity in 214 aa overlap). Contains PS00197 2Fe-2S ferredoxins, iron-sulfur binding region signature. NOTE THAT SUCCINATE DEHYDROGENASE FORMS GENERALLY PART OF AN ENZYME COMPLEX CONTAINING FOUR SUBUNITS: A FLAVOPROTEIN (Rv0248c ?), AN IRON-SULFUR (Rv0247c ?), AND TWO HYDROPHOBIC ANCHOR PROTEINS (Rv0249c ?). Protein product from Mb0253c detected using shotgun mass spectrometry. Mb0253c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." /experiment="experimental evidence, no additional details recorded" BEGIN 1 MTYSASMRVW RGDESCGELR EFTVEVNEGE VVLDVILRLQ QTQTPDLAVR WNCKAGKCGS 61 CSAEINGKPR LMCMTRMSTF DEDEIVTVTP MRTFPVIRDL VTDVSFNYQK AREIPSFAPP 121 KELQPSEYRM AQVDVARSQE FRKCIECFLC QNVCHVVRDH EENKDAFAGP RFLMRIAELE 181 MHPLDTRDRR SQAQEEHGLG YCNITKCCTE VCPENIKITD NALIPMKERV ADRKYDPVVW 241 LGSKLFRR //