LOCUS CAB5247955.1 440 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ACETYL-COA ACYLTRANSFERASE FADA2 (3-KETOACYL-COA THIOLASE) (BETA-KETOTHIOLASE) protein. ACCESSION LT708304-254 PROTEIN_ID CAB5247955.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="fadA2" /locus_tag="BQ2027_MB0249" /note="Mb0249, fadA2, len: 440 aa. Equivalent to Rv0243, len: 440 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 440 aa overlap). Probable fadA2, acetyl-CoA acyltransferase (3-acyl-CoA thiolase) (EC 2.3.1.16), equivalent, but shorter 17 aa, to AL022486|MLCB1883_14T44879 acetyltransferase from Mycobacterium leprae (457 aa), FASTA scores: opt: 250 7, E(): 0, (87.6% identity in 435 aa overlap). Also highly similar to many e.g. G83046|PA478 probable acyl-CoA thiolase from Pseudomonas aeruginosa (425 aa); AB77293.1|AL160312 putative ketoacyl CoA thiolase from Streptomyces coelicolor (428 aa); P76503|7449731|YFCY_ECOLI|D65007|B2342 PROBABLE 3-KETOACYL-COA THIOLASE (ACETYL-COA ACYLTRANSFERASE) (BETA-KETOTHIOLASE) from Escherichia coli strain K-12 (436 aa), FASTA scores: opt: 914, E(): 0, (38.2% identity in 434 aa overlap); P55084|ECHB_HUMAN MITOCHONDRIAL TRIFUNCTONAL ENZYME (474 aa), FASTA scores: opt: 881, E(): 0, (37.7 identity in 451 aa overlap). Contains PS00099 Thiolases active site. BELONGS TO THE THIOLASE FAMILY. Protein product from Mb0249 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0249 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." BEGIN 1 MAPAAKNTSQ TRRRVAVLGG NRIPFARSDG AYADASNQDM FTAALSGLVD RFGLAGERLD 61 MVVGGAVLKH SRDFNLMREC VLGSELSPYT PAFDLQQACG TGLQAAIAAA DGIAAGRYEV 121 AAAGGVDTTS DPPIGLGDDL RRTLLKLRRS RSNVQRLKLV GTLPASLGVE IPANSEPRTG 181 LSMGEHAAVT AKQMGIKRVD QDELAAASHR NMADAYDRGF FDDLVSPFLG LYRDDNLRPN 241 SSVEKLATLR PVFGVKAGDA TMTAGNSTPL TDGASVALLA SEQWAEAHSL APLAYLVDAE 301 TAAVDYVNGN DGLLMAPTYA VPRLLARNGL SLQDFDFYEI HEAFASVVLA HLAAWESEEY 361 CKRRLGLDAA LGSIDRSKLN VNGSSLAAGH PFAATGGRIL AQTAKQLAEK KAAKKGGGPL 421 RGLISICAAG GQGVAAILEA //