LOCUS       CAB5247955.1             440 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE
            ACETYL-COA ACYLTRANSFERASE FADA2 (3-KETOACYL-COA THIOLASE)
            (BETA-KETOTHIOLASE) protein.
ACCESSION   LT708304-254
PROTEIN_ID  CAB5247955.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /gene="fadA2"
                     /locus_tag="BQ2027_MB0249"
                     /note="Mb0249, fadA2, len: 440 aa. Equivalent to Rv0243,
                     len: 440 aa, from Mycobacterium tuberculosis strain H37Rv,
                     (100.0% identity in 440 aa overlap). Probable fadA2,
                     acetyl-CoA acyltransferase (3-acyl-CoA thiolase) (EC
                     2.3.1.16), equivalent, but shorter 17 aa, to
                     AL022486|MLCB1883_14T44879 acetyltransferase from
                     Mycobacterium leprae (457 aa), FASTA scores: opt: 250 7,
                     E(): 0, (87.6% identity in 435 aa overlap). Also highly
                     similar to many e.g. G83046|PA478 probable acyl-CoA
                     thiolase from Pseudomonas aeruginosa (425 aa);
                     AB77293.1|AL160312 putative ketoacyl CoA thiolase from
                     Streptomyces coelicolor (428 aa);
                     P76503|7449731|YFCY_ECOLI|D65007|B2342 PROBABLE
                     3-KETOACYL-COA THIOLASE (ACETYL-COA ACYLTRANSFERASE)
                     (BETA-KETOTHIOLASE) from Escherichia coli strain K-12 (436
                     aa), FASTA scores: opt: 914, E(): 0, (38.2% identity in
                     434 aa overlap); P55084|ECHB_HUMAN MITOCHONDRIAL
                     TRIFUNCTONAL ENZYME (474 aa), FASTA scores: opt: 881, E():
                     0, (37.7 identity in 451 aa overlap). Contains PS00099
                     Thiolases active site. BELONGS TO THE THIOLASE FAMILY.
                     Protein product from Mb0249 detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb0249 found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing (TPM > 20)."
BEGIN
        1 MAPAAKNTSQ TRRRVAVLGG NRIPFARSDG AYADASNQDM FTAALSGLVD RFGLAGERLD
       61 MVVGGAVLKH SRDFNLMREC VLGSELSPYT PAFDLQQACG TGLQAAIAAA DGIAAGRYEV
      121 AAAGGVDTTS DPPIGLGDDL RRTLLKLRRS RSNVQRLKLV GTLPASLGVE IPANSEPRTG
      181 LSMGEHAAVT AKQMGIKRVD QDELAAASHR NMADAYDRGF FDDLVSPFLG LYRDDNLRPN
      241 SSVEKLATLR PVFGVKAGDA TMTAGNSTPL TDGASVALLA SEQWAEAHSL APLAYLVDAE
      301 TAAVDYVNGN DGLLMAPTYA VPRLLARNGL SLQDFDFYEI HEAFASVVLA HLAAWESEEY
      361 CKRRLGLDAA LGSIDRSKLN VNGSSLAAGH PFAATGGRIL AQTAKQLAEK KAAKKGGGPL
      421 RGLISICAAG GQGVAAILEA
//