LOCUS CAB5247944.1 314 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 ribonucleoside- diphosphate reductase (beta chain) nrdb (ribonucleotide reductase small chain) protein. ACCESSION LT708304-243 PROTEIN_ID CAB5247944.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="nrdB" /locus_tag="BQ2027_MB0238" /note="Mb0238, nrdB, len: 314 aa. Equivalent to Rv0233, len: 314 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 314 aa overlap). Probable nrdB (alternate gene name: rnrS) ribonucleoside-diphosphate reductase, beta chain (EC 1.17.4.1), similar to others e.g. RIR2_SCHPO|P36603 ribonucleoside-diphosphate reductase (391 aa), FASTA scores: opt: 168, E(): 0.00018, (26.1% identity in 199 aa overlap); etc. BELONGS TO THE RIBONUCLEOSIDE DIPHOSPHATE REDUCTASE SMALL CHAIN FAMILY. COFACTOR: BINDS 2 IRON IONS. Protein product from Mb0238 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0238 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MTRTRSGSLA AGGLNWASLP LKLFAGGNAK FWDPADIDFT RDRADWEKLS DDERDYATRL 61 CTQFIAGEEA VTEDIQPFMS AMRAEGRLAD EMYLTQFAFE EAKHTQVFRM WLDAVGISED 121 LHRYLDDLPA YRQIFYAELP ECLNALSADP SPAAQVRASV TYNHIVEGML ALTGYYAWHK 181 ICVERAILPG MQELVRRIGD DERRHMAWGT FTCRRHVAAD DANWTVFETR MNELIPLALR 241 LIEEGFALYG DQPPFDLSKD DFLQYSTDKG MRRFGTISNA RGRPVAEIDV DYSPAQLEDT 301 FADEDRRTLA AASA //