LOCUS CAB5247934.1 385 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE ALDEHYDE DEHYDROGENASE protein. ACCESSION LT708304-233 PROTEIN_ID CAB5247934.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0228C" /note="Mb0228c, -, len: 385 aa. Equivalent to Rv0223c, len: 487 aa, from Mycobacterium tuberculosis strain H37Rv, (95.3% identity in 364 aa overlap). Probable aldehyde dehydrogenase (EC 1.2.1.-), similar to others e.g. A75608|6460525|AAF12231.1|AE001862_57 aldehyde dehydrogenase from Deinococcus radiodurans strain R1 (495 aa); Q47943 L-sorbosone dehydrogenase NAD(P) dependent from Gluconobacter oxydans (498 aa), FASTA scores: opt: 1157, E (): 0, (42.1% identity in 482 aa overlap); etc. Also similar to Rv0768, Rv2858c, etc from Mycobacterium tuberculosis. Contains PS00687 Aldehyde dehydrogenases glutamic acid active site; and PS00070 Aldehyde dehydrogenases cysteine active site. BELONGS TO THE ALDEHYDE DEHYDROGENASES FAMILY. REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, a single base insertion (*-c) leads to a shorter product, with a different COOH part compared to its homolog in Mycobacterium tuberculosis strain H37Rv (385 aa versus 487 aa). Protein product from Mb0228c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0228c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." BEGIN 1 MSDSATEYDK LFIGGKWTKP STSDVIEVRC PATGEYVGKV PMAAAADVDA AVAAARAAFD 61 NGPWPSTPPH ERAAVIAAAV KMLAERKDLF TKLLAAETGQ PPTIIETMHW MGSMGAMNYF 121 AGAADKVTWT ETRTGSYGQS IVSRESVGVV GAIVAWNVPL FLAVNKIAPA LLAGCTIVLK 181 PAAETPLTAN ALAEVFAEVG LPEGVLSVVP GGIETGQALT SNPDIDMFTF TGSSAVGREV 241 GRRAAEMLKP CTLELGGKSA AIILEDVDLA AAIPMMVFSG VMNAGQGCVN QTRILAPRSR 301 YDEIVAAVTN FVTALPVGPP SDPAAQIGPL ISEKQRTRVE ALHRQGHRGG RSVGVRRRPS 361 RGLGQRLLYP IHERRWRGKW HGQCG //