LOCUS CAB5247933.1 257 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 possible triacylglycerol synthase (diacylglycerol acyltransferase) protein. ACCESSION LT708304-232 PROTEIN_ID CAB5247933.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0227" /note="Mb0227, -, len: 257 aa. Equivalent to 5' end of Rv0221, len: 469 aa, from Mycobacterium tuberculosis strain H37Rv, (98.3% identity in 234 aa overlap). Conserved hypothetical protein, similar to others proteins from Mycobacterium tuberculosis e.g. Q50680|Rv2285|MT2343|MTCY339.25c hypothetical 47.7 kDa protein (445 aa), FASTA scores: opt: 455, E(): 8.1e-23, (26.7% identity in 461 aa overlap); Rv3740c, Rv3734c, etc. REMARK-M.bovis-M.tuberculosis: In Mycobacterium bovis, a 1902 bp deletion leads to a shorter product with a different COOH part, compared to its homolog in Mycobacterium tuberculosis strain H37Rv (257 aa versus 469 aa). It also leads to the deletion of the next protein, echA1 (Rv0222). Protein product from Mb0227 detected using SWATH mass spectrometry. Mb0227 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MKRLSGWDAV LLYSETPNVH MHTLKVAVIE LDSDRQEFGV DAFREVIAGR LHKLEPLGYQ 61 LVDVPLKFHH PMWREHCQVD LNYHIRPWRL RAPGGRRELD EAVGEIASTP LNRDHPLWEM 121 YFVEGLANHR IAVVAKIHHA LADGVASANM MARGMDLLPG PEVGRYVPDP APTKRQLLSA 181 AFIDHLRHLG RIPATIRYTT QGLGRVRRSS RKLSPALTMP FTPPPTFMNR IKKPLSKPSG 241 RPPPHTNRAP SSMPLAM //