LOCUS CAB5247930.1 442 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CONSERVED TRANSMEMBRANE PROTEIN protein. ACCESSION LT708304-229 PROTEIN_ID CAB5247930.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0224" /note="Mb0224, -, len: 442 aa. Equivalent to Rv0218, len: 442 aa, from Mycobacterium tuberculosis strain H37Rv, (99.5% identity in 442 aa overlap). Probable conserved transmembrane protein, some similarity with sulfite oxidases (EC 1.8.3.1) e.g. SUOX_HUMAN|P51687 sulfite oxidase precursor (488 aa), FASTA scores: opt: 153, E(): 0.0087, (28.6% identity in 161 aa overlap); and with some nitrate reductases (EC 1.6.6.3) e.g. NIA_FUSOX|P39863 nitrate reductase (NADPH) (905 aa), FASTA scores: opt: 143, E(): 0.06, (29.3% identity in 92 aa overlap). Also similar to BSUB0017_86 from Mycobacterium tuberculosis. Mb0224 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MSDPARGAEA EDAYGFPAGL WRWLQRHPPP ALHRLTRFRS PLRGPWLTSV FGLVLLVALP 61 FVIITGLLSY IAYAPQLGQA IPGDVGWLRL PAFTWPTRPS WLYRLTQGLH VGLGLVIIPV 121 VLAKLWSVIP RLFVWPPARS IAQVLERLSV LMLVGGILFQ IVTGVLNIQY DYIFGFSFYT 181 GHYFGAWVFI AGFLLHIVVK IPHMVTGLRS IPMREVLGTN VADTRAQPCD PDGLVSVNPG 241 EATLSRRGAL GLVGAGVLLI GVLTVGQTLG GFTRKAALLL PRGRVVSPGD FPVNKTAAAA 301 GITAEAIGPD WRLVLRGGPA EVVLDRATLA GLPQRTARLP LACVEGWSAV RTWSGVPLAE 361 LALLAGVPAA RSARVTSLQR GGAFGEAKLA ANQIADPDAL LALRVDGADL SLNHGYPARI 421 IVPALPGVHN TKWVAGIEFH KR //