LOCUS CAB5247929.1 302 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE ESTERASE LIPW protein. ACCESSION LT708304-228 PROTEIN_ID CAB5247929.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="lipW" /locus_tag="BQ2027_MB0223C" /note="Mb0223c, lipW, len: 302 aa. Equivalent to Rv0217c, len: 302 aa, from Mycobacterium tuberculosis strain H37Rv, (99.7% identity in 302 aa overlap). Possible esterase (EC 3.1.1.-), showing similarity with others e.g. EST_ACICA|P18773 esterase (303 aa), FASTA scores: opt: 320, E(): 3.2e-13, (29.2% identity in 274 aa overlap). Protein product from Mb0223c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0223c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MSGNEVHPDL RRIAVVTPRQ LVGPRTLPVM RALIVVAGLR MSRTPPDIEV LTLESGVGVR 61 LYRPAGSNEP APALLWIHAG GYVMGTAQQD DRLCLRFSSR LGITVASVDY RLAPENPYPA 121 ALGDCYSALT WLASLPAVDP ARVAIGGASA GGGLAAALAL LARDRGGITP AFQLLVYPML 181 DDRTSIAPAN PHYRLWNGRA NRFGWRAYLG DADARVAVPG RRDDLGGLAP AWIGVGTHDL 241 LHDEDLAYAE RLTAAGVPCQ VEVVEGAFHG FDRVAPNVGV SQRFFTSQCN SLRAALALSN 301 RT //