LOCUS CAB5247928.1 337 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 double hotdog hydratase protein. ACCESSION LT708304-227 PROTEIN_ID CAB5247928.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0222" /note="Mb0222, -, len: 337 aa. Equivalent to Rv0216, len: 337 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 337 aa overlap). Conserved hypothetical protein, equivalent to Z95398|MLCL622_22 from Mycobacterium leprae (339 aa), FASTA scores: E(): 0, (73.7 identity in 338 aa overlap). Protein product from Mb0222 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0222 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MASGYGGIRV GGPYFDDLSK GQVFDWAPGV TLSLGLAAAH QSIVGNRLRL ALDSDLCAAV 61 TGMPGPLAHP GLVCDVAIGQ STLATQRVKA NLFYRGLRFH RFPAVGDTLY TRTEVVGLRA 121 NSPKPGRAPT GLAGLRMTTI DRTDRLVLDF YRCAMLPASP DWKPGAVPGD DLSRIGADAP 181 APAADPTAHW DGAVFRKRVP GPHFDAGIAG AVLHSTADLV SGAPELARLT LNIAATHHDW 241 RVSGRRLVYG GHTIGLALAQ ATRLLPNLAT VLDWESCDHT APVHEGDTLY SELHIESAQA 301 HADGGVLGLR SLVYAVSDSA SEPDRQVLDW RFSALQF //