LOCUS CAB5247925.1 437 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 POSSIBLE METHYLTRANSFERASE (METHYLASE) protein. ACCESSION LT708304-224 PROTEIN_ID CAB5247925.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0219C" /note="Mb0219c, -, len: 437 aa. Equivalent to Rv0213c, len: 437 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 437 aa overlap). Possible methyltransferase (EC 2.1.1.-), weakly similar to others methyltransferases e.g. AF127374_30|LINA from Streptomyces lavendulae (611 aa), FASTA scores: opt: 400, E(): 8.1e-19, (27.3% identity in 388 aa overlap); Q50258 fortimicin kl1 methyltransferase (553 aa), FASTA scores: opt: 267, E(): 1.2e-13, (29.3% identity in 351 aa overlap). Mb0219c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing (TPM > 20)." BEGIN 1 MSIKAYAKTQ GIAVTSVNGL VAGHGSVQET WLAMQSAAAL SGTPRLVGFS CIDTFPEVLW 61 LAQRARQAWD GVRIVIGNAM ATLNYERILR QHDCFDYVVV GDGEVAFTKL ALALANDAAV 121 DDVPGLARRS EQGQILRTPS SLVDLDELPR PARDELPTVL ADGFAASVFS TRGCPYRCTF 181 CGTGAMSAML GKDSYRAKSV DAVVDEIDYL VSDYDVNFLS ITDDLFISKH PGSQQRAADF 241 ANAVLRRGIS VNFMVDIRLD SVVDLDLFKH LHRAGLRRVF IGVETGSYEQ LRAYRKQILT 301 RGQDAADTIN ALQQLGIDVI PGTIMFHPTV QPDELRETVR LLRATKYTVG FKFMSRIVPY 361 PGTPLYQAYS DAGYLTAKWP LGQWEFVDPE ASRVYADVVA KVAPDVGISF DEAEAYFLSR 421 LDEWENVIAG RIAEATS //