LOCUS CAB5247910.1 663 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 probable zinc metalloprotease zmp1 protein. ACCESSION LT708304-209 PROTEIN_ID CAB5247910.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /gene="zmp1" /locus_tag="BQ2027_MB0204C" /note="Mb0204c, -, len: 663 aa. Equivalent to Rv0198c, len: 663 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 663 aa overlap). Probable zinc metalloprotease (EC 3.4.24.-), equivalent to Z95398|MLCL622.12c from Mycobacterium leprae (667 aa), FASTA scores: opt: 3710, E(): 0, (80.8 % identity in 667 aa overlap). Also similar to many other metalloproteases e.g. members of the eukaryotic neprilysin family: P08473|NEP_HUMAN NEPRILYSIN (EC 3.4.24.11) (749 aa), FASTA scores: opt: 872, E(): 0, (31.1% identity in 692 aa overlap); Q07744|PEPO_LACLA NEUTRAL ENDOPEPTIDASE from Lactococcus lactis (626 aa), FASTA scores: opt: 862, E(): 0, (30.0% identity in 654 aa overlap). Contains PS00142 Neutral zinc metallopeptidases, zinc-binding region signature. BELONGS TO PEPTIDASE FAMILY M13 (ZINC METALLOPROTEASE); ALSO KNOWN AS THE NEPRILYSIN SUBFAMILY. Protein product from Mb0204c detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0204c found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MTLAIPSGID LSHIDADARP QDDLFGHVNG RWLAEHEIPA DRATDGAFRS LFDRAETQVR 61 DLIIQASQAG AAVGTDAQRI GDLYASFLDE EAVERAGVQP LHDELATIDS AADATELAAA 121 LGTLQRAGVG GGIGVYVDTD SKDSTRYLVH FTQSGIGLPD ESYYRDEQHA AVLAAYPGHI 181 ARMFGLVYGG ESRDHAKTAD RIVALETKLA DAHWDVVKRR DADLGYNLRT FAQLQTEGAG 241 FDWVSWVTAL GSAPDAMTEL VVRQPDYLVT FASLWASVNV EDWKCWARWR LIRARAPWLT 301 RALVAEDFEF YGRTLTGAQQ LRDRWKRGVS LVENLMGDAV GKLYVQRHFP PDAKSRIDTL 361 VDNLQEAYRI SISELDWMTP QTRQRALAKL NKFTAKVGYP IKWRDYSKLA IDRDDLYGNV 421 QRGYAVNHDR ELAKLFGPVD RDEWFMTPQT VNAYYNPGMN EIVFPAAILQ PPFFDPQADE 481 AANYGGIGAV IGHEIGHGFD DQGAKYDGDG NLVDWWTDDD RTEFAARTKA LIEQYHAYTP 541 RDLVDHPGPP HVQGAFTIGE NIGDLGGLSI ALLAYQLSLN GNPAPVIDGL TGMQRVFFGW 601 AQIWRTKSRA AEAIRRLAVD PHSPPEFRCN GVVRNVDAFY QAFDVTEDDA LFLDPQRRVR 661 IWN //