LOCUS       CAB5247904.1             352 aa    PRT              BCT 25-MAY-2020
DEFINITION  Mycobacterium tuberculosis variant bovis AF2122/97 L,D-
            transpeptidase protein.
ACCESSION   LT708304-203
PROTEIN_ID  CAB5247904.1
SOURCE      Mycobacterium tuberculosis variant bovis AF2122/97
  ORGANISM  Mycobacterium tuberculosis variant bovis AF2122/97
            Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
            Mycobacterium; Mycobacterium tuberculosis complex.
REFERENCE   1
  AUTHORS   Malone K.M.
  JOURNAL   Submitted (06-DEC-2016) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine, University College
            Dublin, D4, Ireland
REFERENCE   2
  AUTHORS   Malone M K., Farrell D., Malone K.
  JOURNAL   Submitted (15-APR-2020) to the INSDC. School of Veterinary
            Medicine, Tuberculosis Molecular Microbiology Research Group,
            University College Dublin, Tuberculosis Molecular Microbiology
            Research Group, School of Veterinary Medicine,, University College
            Dublin, D4, Ireland
FEATURES             Qualifiers
     source          /organism="Mycobacterium tuberculosis variant bovis
                     AF2122/97"
                     /chromosome="Mycobacterium_bovis_AF212297"
                     /isolate="AF2122/97"
                     /mol_type="genomic DNA"
                     /isolation_source="Mycobacterium bovis subsp. bovis strain
                     AF2122/97. This strain is a fully virulent strain that was
                     isolated in 1997 in the UK from a cow suffering necrotic
                     lesions in lung and bronchomediastinal lymph nodes. The
                     strain was also reported to infect and persist in badgers
                     that are considered to be a significant source of bovine
                     infection."
                     /db_xref="taxon:233413"
     protein         /transl_table=11
                     /locus_tag="BQ2027_MB0198"
                     /note="Mb0198, -, len: 352 aa. Equivalent to Rv0192 and
                     Rv0192A, len: 366 aa and 100 aa, from Mycobacterium
                     tuberculosis strain H37Rv, (96.5% identity in 318 aa
                     overlap and 91.1% identity in 56 aa overlap). Conserved
                     hypothetical protein. Has Gly- Arg-rich region followed by
                     highly Pro-rich repetitive region near N-terminus. Similar
                     in C-terminus to other hypothetical proteins e.g.
                     Q49706|B1496_F2_81|U00013 from Mycobacterium leprae (271
                     aa), FASTA scores: opt: 375, E(): 3.2e-24, (36.1% identity
                     in 255 aa overlap); YV09_MYCTU|Q11149|cY20G9.09
                     hypothetical 47.9 kd protein from Mycobacterium
                     tuberculosis (451 aa), FASTA scores: opt: 330, E():
                     3.2e-13, (35.1% identity in 271 aa overlap). Also similar
                     to Rv0116c, Rv1433, Rv2518c, Rv0483 from Mycobacterium
                     tuberculosis. Probable N-terminal part of Rv0192, which is
                     member of family P5.17 with Rv0116c, Rv1433, Rv2518c,
                     Rv0483. These are all predicted to be exported/membrane
                     proteins. Rv0192A has typical N-terminal signal peptide
                     which is functional and was identified by PhoA fusion
                     screens: O52054 PGB14T-O1 PRECURSOR (FRAGMENT 45 AA) (see
                     citation below). Since Rv0192 misses a signal peptide this
                     suggests that there is a frameshift in the region of the
                     overlap with Rv0192 but none found on reinspection of
                     sequence. REMARK-M.bovis-M.tuberculosis: In Mycobacterium
                     tuberculosis strain H37Rv, Rv0192 and Rv0192A exist as 2
                     genes with an overlap region. In Mycobacterium bovis, a
                     single base insertion (*-g) leads to a single product.
                     Protein product from Mb0198 detected using shotgun mass
                     spectrometry and SWATH mass spectrometry. Mb0198 found to
                     be expressed during exponential growth in Sauton's minimal
                     media by RNA-sequencing."
BEGIN
        1 MSRWKQGWTR GSLFAALNIA AVVAVLMLGA GVAVADPDAA PGDPGGPGGP GGTAGPVDPP
       61 AVDLLAPPPD PLALPPALDP LAPPPPDPLA PPPPDPLAVP VAAGPVAGQD PTPFVGPPPF
      121 RPPTFNPVDG AMVGVAKPIV INFAVPIADR AMAESAIHIS SIPPVPGKFY WMSPTQVRWR
      181 PFEFWPANTA VNIDAAGTKS SFRTGDSLVA TADDATHQMT ITRNGVVQKT FPMSMGMVSG
      241 GHQTPNGTYY VLEKFATVVM DSSTYGVPVN SAQGYKLTVS DAVRIDNSGN FVHSAPWSVA
      301 DQGKRNVTHG CINLSPANAK WFYDNFGSGD PVVVKNSVGT YNKNDGAQDW QI
//