LOCUS CAB5247904.1 352 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 L,D- transpeptidase protein. ACCESSION LT708304-203 PROTEIN_ID CAB5247904.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0198" /note="Mb0198, -, len: 352 aa. Equivalent to Rv0192 and Rv0192A, len: 366 aa and 100 aa, from Mycobacterium tuberculosis strain H37Rv, (96.5% identity in 318 aa overlap and 91.1% identity in 56 aa overlap). Conserved hypothetical protein. Has Gly- Arg-rich region followed by highly Pro-rich repetitive region near N-terminus. Similar in C-terminus to other hypothetical proteins e.g. Q49706|B1496_F2_81|U00013 from Mycobacterium leprae (271 aa), FASTA scores: opt: 375, E(): 3.2e-24, (36.1% identity in 255 aa overlap); YV09_MYCTU|Q11149|cY20G9.09 hypothetical 47.9 kd protein from Mycobacterium tuberculosis (451 aa), FASTA scores: opt: 330, E(): 3.2e-13, (35.1% identity in 271 aa overlap). Also similar to Rv0116c, Rv1433, Rv2518c, Rv0483 from Mycobacterium tuberculosis. Probable N-terminal part of Rv0192, which is member of family P5.17 with Rv0116c, Rv1433, Rv2518c, Rv0483. These are all predicted to be exported/membrane proteins. Rv0192A has typical N-terminal signal peptide which is functional and was identified by PhoA fusion screens: O52054 PGB14T-O1 PRECURSOR (FRAGMENT 45 AA) (see citation below). Since Rv0192 misses a signal peptide this suggests that there is a frameshift in the region of the overlap with Rv0192 but none found on reinspection of sequence. REMARK-M.bovis-M.tuberculosis: In Mycobacterium tuberculosis strain H37Rv, Rv0192 and Rv0192A exist as 2 genes with an overlap region. In Mycobacterium bovis, a single base insertion (*-g) leads to a single product. Protein product from Mb0198 detected using shotgun mass spectrometry and SWATH mass spectrometry. Mb0198 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MSRWKQGWTR GSLFAALNIA AVVAVLMLGA GVAVADPDAA PGDPGGPGGP GGTAGPVDPP 61 AVDLLAPPPD PLALPPALDP LAPPPPDPLA PPPPDPLAVP VAAGPVAGQD PTPFVGPPPF 121 RPPTFNPVDG AMVGVAKPIV INFAVPIADR AMAESAIHIS SIPPVPGKFY WMSPTQVRWR 181 PFEFWPANTA VNIDAAGTKS SFRTGDSLVA TADDATHQMT ITRNGVVQKT FPMSMGMVSG 241 GHQTPNGTYY VLEKFATVVM DSSTYGVPVN SAQGYKLTVS DAVRIDNSGN FVHSAPWSVA 301 DQGKRNVTHG CINLSPANAK WFYDNFGSGD PVVVKNSVGT YNKNDGAQDW QI //