LOCUS CAB5247903.1 413 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE CONSERVED INTEGRAL MEMBRANE PROTEIN protein. ACCESSION LT708304-202 PROTEIN_ID CAB5247903.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0197" /note="Mb0197, -, len: 413 aa. Equivalent to Rv0191, len: 413 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 413 aa overlap). Probable conserved integral membrane protein, member of major facilitator superfamily (MFS) possibly involved in transport of drug, similar to several hypothetical proteins e.g. YDEA_ECOLI|P31122 hypothetical 42.5 kd protein from Escherichia coli (396 aa), FASTA scores: opt: 475, E(): 4.2e-33, (29.7% identity in 381 aa overlap); and to several chloramphenicol resistance proteins e.g. CMLR_STRLI|P31141 chloramphenicol resistance protein from stremtomyces lividans (392 aa), FASTA scores: opt: 394, E(): 6.7e-12, (28.2% identity in 383 aa overlap). Also similar to SVU09991_1 from Mycobacterium tuberculosis. Protein product from Mb0197 detected using SWATH mass spectrometry. Mb0197 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MTAPTGTSAT TTRPWTPRIA TQLSVLACAA FIYVTAEILP VGALSAIARN LRVSVVLVGT 61 LLSWYALVAA VTTVPLVRWT AHWPRRRALV VSLVCLTVSQ LVSALAPNFA VLAAGRVLCA 121 VTHGLLWAVI APIATRLVPP SHAGRATTSI YIGTSLALVV GSPLTAAMSL MWGWRLAAVC 181 VTGAAAAVAL AARLALPEMV LRADQLEHVG RRARHHRNPR LVKVSVLTMI AVTGHFVSYT 241 YIVVIIRDVV GVRGPNLAWL LAAYGVAGLV SVPLVARPLD RWPKGAVIVG MTGLTAAFTL 301 LTALAFGERH TAATALLGTG AIVLWGALAT AVSPMLQSAA MRSGGDDPDG ASGLYVTAFQ 361 IGIMAGALLG GLLYERSLAM MLTASAGLMG VALFGMTVSQ HLFENPTLSP GDG //