LOCUS CAB5247899.1 220 aa PRT BCT 25-MAY-2020 DEFINITION Mycobacterium tuberculosis variant bovis AF2122/97 PROBABLE O-METHYLTRANSFERASE protein. ACCESSION LT708304-198 PROTEIN_ID CAB5247899.1 SOURCE Mycobacterium tuberculosis variant bovis AF2122/97 ORGANISM Mycobacterium tuberculosis variant bovis AF2122/97 Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. REFERENCE 1 AUTHORS Malone K.M. JOURNAL Submitted (06-DEC-2016) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine, University College Dublin, D4, Ireland REFERENCE 2 AUTHORS Malone M K., Farrell D., Malone K. JOURNAL Submitted (15-APR-2020) to the INSDC. School of Veterinary Medicine, Tuberculosis Molecular Microbiology Research Group, University College Dublin, Tuberculosis Molecular Microbiology Research Group, School of Veterinary Medicine,, University College Dublin, D4, Ireland FEATURES Qualifiers source /organism="Mycobacterium tuberculosis variant bovis AF2122/97" /chromosome="Mycobacterium_bovis_AF212297" /isolate="AF2122/97" /mol_type="genomic DNA" /isolation_source="Mycobacterium bovis subsp. bovis strain AF2122/97. This strain is a fully virulent strain that was isolated in 1997 in the UK from a cow suffering necrotic lesions in lung and bronchomediastinal lymph nodes. The strain was also reported to infect and persist in badgers that are considered to be a significant source of bovine infection." /db_xref="taxon:233413" protein /transl_table=11 /locus_tag="BQ2027_MB0193" /note="Mb0193, -, len: 220 aa. Equivalent to Rv0187, len: 220 aa, from Mycobacterium tuberculosis strain H37Rv, (100.0% identity in 220 aa overlap). Probable O-methyltransferase (EC 2.1.1.-), similar to many e.g. AB93458.1|AL357591 putative O-methyltransferase from Streptomyces coelicolor (223 aa); MDMC_STRMY|Q00719 O-methyltransferase from Streptomyces mycarofaciens (221 aa), FASTA scores: opt: 327, E(): 2.4e-17, (35.9% identity in 192 aa overlap). Also similar to Rv1703c, Rv1220c from Mycobacterium tuberculosis. Protein product from Mb0193 detected using shotgun mass spectrometry. Mb0193 found to be expressed during exponential growth in Sauton's minimal media by RNA-sequencing." BEGIN 1 MGMDQQPNPP DVDAFLDSTL VGDDPALAAA LAASDAAELP RIAVSAQQGK FLCLLAGAIQ 61 ARRVLEIGTL GGFSTIWLAR GAGPQGRVVT LEYQPKHAEV ARVNLQRAGV ADRVEVVVGP 121 ALDTLPTLAG GPFDLVFIDA DKENNVAYIQ WAIRLARRGA VIVVDNVIRG GGILAESDDA 181 DAVAARRTLQ MMGEHPGLDA TAIQTVGRKG WDGFALALVR //